Literature summary extracted from

Anderson, W.A.; Moo-Young, M.; Legge, R.L.
Development of a multienzyme reactor for dopamine synthesis: I. Enzymology and kinetics (1992), Biotechnol. Bioeng., 39, 781-789.

Application

EC Number	Application	Comment	Organism
4.1.99.2	synthesis	development of a multienzyme reactor with ec 4.1.99.2 and ec 4.1.1.25 for dopamine synthesis	Pantoea agglomerans

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.1.99.2	40	-	catechol	-	Pantoea agglomerans
4.1.99.2	110	-	pyruvate	-	Pantoea agglomerans
4.1.99.2	250	-	NH3	-	Pantoea agglomerans

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.1.99.2	Pantoea agglomerans	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.1.99.2	3,4-dihydroxyphenyl-L-Ala + H2O	-	Pantoea agglomerans	pyrocatechol + NH3 + pyruvate	-	?
4.1.99.2	catechol + pyruvate + NH3	-	Pantoea agglomerans	L-dihydroxyphenylalanine	-	?
4.1.99.2	pyrocatechol + pyruvate + NH3	-	Pantoea agglomerans	3,4-dihydroxyphenyl-L-Ala + H2O	-	?

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Release 2023.1 (January 2023)