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Literature summary extracted from

  • Qian, N.; Stanley, G.A.; Hahn-Hägerdal, B.; Radström, P.
    Purification and characterization of two phosphoglucomutases from Lactococcus lactis subsp. lactis and their regulation in maltose- and glucose-utilizing cells (1994), J. Bacteriol., 176, 5304-5311.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.2.2 alpha-D-glucose 1,6-diphosphate required Lactococcus lactis
5.4.2.6 beta-D-Glucose 1,6-diphosphate required Lactobacillus delbrueckii subsp. lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.2.2 Mg2+ required Lactococcus lactis
5.4.2.6 Mg2+ required Lactobacillus delbrueckii subsp. lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.2.2 65000
-
 gel filtration, SDS-PAGE, zymogram analysis Lactococcus lactis
5.4.2.6 25000
-
 1 * 25000, SDS-PAGE Lactobacillus delbrueckii subsp. lactis
5.4.2.6 28000
-
 gel filtration Lactobacillus delbrueckii subsp. lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.2.2 alpha-D-glucose 1-phosphate Lactococcus lactis degradation of lactose in lactic acid bacteria ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.2 Lactococcus lactis
-
 subsp. lactis
-
5.4.2.6 Lactobacillus delbrueckii subsp. lactis
-
 subsp. lactis, strains 19435 and 65.1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.2 partial Lactococcus lactis
5.4.2.6
-
 Lactobacillus delbrueckii subsp. lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.2.2 additional information
-
-
 Lactococcus lactis
5.4.2.6 additional information
-
-
 Lactobacillus delbrueckii subsp. lactis

Storage Stability

EC Number Storage Stability Organism
5.4.2.6 -80°C, partially purified enzyme, stable for at least 3 months Lactobacillus delbrueckii subsp. lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.2 alpha-D-glucose 1-phosphate
-
 Lactococcus lactis alpha-D-glucose 6-phosphate
-
 ?
5.4.2.2 alpha-D-glucose 1-phosphate degradation of lactose in lactic acid bacteria Lactococcus lactis ?
-
 ?
5.4.2.6 beta-D-Glucose 1-phosphate r Lactobacillus delbrueckii subsp. lactis beta-D-Glucose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.2.2 monomer 1 * 65000, SDS-PAGE Lactococcus lactis
5.4.2.6 monomer 1 * 25000, SDS-PAGE Lactobacillus delbrueckii subsp. lactis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.2.2 20
-
 12 h, 30% loss of activity Lactococcus lactis
5.4.2.2 50
-
 15 min, 97% loss of activity Lactococcus lactis
5.4.2.6 22
-
 overnight at room temperature, 50% loss of activity Lactobacillus delbrueckii subsp. lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.2.6 6.3 6.7
-
 Lactobacillus delbrueckii subsp. lactis