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Literature summary extracted from

  • Antranikian, G.; Klinner, C.; Kuemmel, A.; Schwanitz, D.; Zimmermann, T.; Mayer, F.; Gottschalk, G.
    Purification of L-glutamate-dependent citrate lyase from Clostridium sphenoides and electron microscopic analysis of citrate lyase isolated from Rhodopseudomonas gelatinosa, Streptococcus diacetilactis and C. sphenoides (1982), Eur. J. Biochem., 126, 35-42.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.3.6 L-Glu activity is strictly dependent on presence of L-Glu Lacrimispora sphenoides

General Stability

EC Number General Stability Organism
4.1.3.6 only stable in presence of 150 mM L-Glu or 7 mM L-Glu plus glycerol, sucrose or bovine serum albumin Lacrimispora sphenoides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.6 0.12
-
 citrate
-
 Lacrimispora sphenoides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.6 Co2+ strict requirement for a divalent metal ion, Mg2+, Mn2+ or Co2+ Lacrimispora sphenoides
4.1.3.6 Mg2+
-
 Lactococcus lactis subsp. lactis
4.1.3.6 Mg2+ strict requirement for a divalent metal ion, Mg2+, Mn2+ or Co2+. Mg2+ is most effective Lacrimispora sphenoides
4.1.3.6 Mn2+
-
 Lactococcus lactis subsp. lactis
4.1.3.6 Mn2+ strict requirement for a divalent metal ion, Mg2+, Mn2+ or Co2+ Lacrimispora sphenoides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.6 515000
-
 gel filtration, sucrose density gradient centrifugation Lacrimispora sphenoides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.6 citrate Lacrimispora sphenoides regulated by concentration of intracellular L-Glu ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.6 Lacrimispora sphenoides
-
-
-
4.1.3.6 Lactococcus lactis subsp. lactis
-
-
-
4.1.3.6 Rubrivivax gelatinosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.6
-
 Lacrimispora sphenoides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.6 additional information
-
-
 Lactococcus lactis subsp. lactis
4.1.3.6 additional information
-
-
 Lacrimispora sphenoides
4.1.3.6 additional information
-
-
 Rubrivivax gelatinosus

Storage Stability

EC Number Storage Stability Organism
4.1.3.6 -20°C, stable for 2 months Lacrimispora sphenoides
4.1.3.6 0°C, 30% glycerol, 40% sucrose or 10 mg per ml bovine serum albumin, 7 mM L-Glu, only some loss of activity after 5 min Lacrimispora sphenoides
4.1.3.6 4°C, stable for 4 days Lacrimispora sphenoides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.6 citrate
-
 Lactococcus lactis subsp. lactis acetate + oxaloacetate
-
 ?
4.1.3.6 citrate
-
 Lacrimispora sphenoides acetate + oxaloacetate
-
 ?
4.1.3.6 citrate
-
 Rubrivivax gelatinosus acetate + oxaloacetate
-
 ?
4.1.3.6 citrate regulated by concentration of intracellular L-Glu Lacrimispora sphenoides ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.6 30
-
 half-life: 4 h Lacrimispora sphenoides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.6 6.5 7
-
 Lacrimispora sphenoides