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Literature summary extracted from

  • Bekal, S.; van Beeumen, J.; Samyn, B.; Garmyn, D.; Henini, S.; Divies, C.; Prevost, H.
    Purification of Leuconostoc mesenteroides citrate lyase and cloning and characterization of the citCDEFG gene cluster (1998), J. Bacteriol., 180, 647-654.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.6 expression of the citCDEFG gene cluster in Escherichia coli. The gene product of citD is the gamma-subunit of citrate lyase. The gene product of citE is the beta-subunit of citrate lyase. The gene product of citF is the alpha-subunit of citrate lyase. The enzyme synthesized in Escherichia coli lacks its prosthetic group and is active only in the presence of acetyl-CoA Leuconostoc mesenteroides
4.1.3.34 expression in Escherichia coli Leuconostoc mesenteroides
6.2.1.22 the gene citC, which encodes the citrate lyase ligase, is expressed as a subunit of the citCDFG cluster in Escherichia coli Leuconostoc mesenteroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.6 14000
-
 x * 14000 + x * 34000 + x * 55000, SDS-PAGE Leuconostoc mesenteroides
4.1.3.6 34000
-
 x * 14000 + x * 34000 + x * 55000, SDS-PAGE Leuconostoc mesenteroides
4.1.3.6 55000
-
 x * 14000 + x * 34000 + x * 55000, SDS-PAGE Leuconostoc mesenteroides
4.1.3.34 33354
-
 x * 33354, calculation from nucleotide sequence Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.34 (3S)-citryl-[acyl-carrier protein] Leuconostoc mesenteroides enzyme is involved in citrate fermentation acetyl-[acyl-carrier protein] + oxaloacetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.6 Leuconostoc mesenteroides
-
-
-
4.1.3.34 Leuconostoc mesenteroides
-
-
-
6.2.1.22 Leuconostoc mesenteroides
-
 component of the citrate lyase cluster
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.6
-
 Leuconostoc mesenteroides
4.1.3.34
-
 Leuconostoc mesenteroides
6.2.1.22 cannot be co-purified with the citrate lyase subunits Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.6 additional information
-
-
 Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.6 citrate
-
 Leuconostoc mesenteroides acetate + oxaloacetate
-
 ?
4.1.3.34 (3S)-citryl-[acyl-carrier protein]
-
 Leuconostoc mesenteroides acetyl-[acyl-carrier protein] + oxaloacetate
-
 ?
4.1.3.34 (3S)-citryl-[acyl-carrier protein] enzyme is involved in citrate fermentation Leuconostoc mesenteroides acetyl-[acyl-carrier protein] + oxaloacetate
-
 ?
6.2.1.22 ATP + acetate + citrate (pro-3S)-lyase (thiol form)
-
 Leuconostoc mesenteroides AMP + diphosphate + citrate(pro-3S)-lyase (acetyl form)
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.6 oligomer x * 14000 + x * 34000 + x * 55000, SDS-PAGE Leuconostoc mesenteroides
4.1.3.34 ? x * 33354, calculation from nucleotide sequence Leuconostoc mesenteroides
4.1.3.34 More the enzyme is the beta-subunit of EC 4.1.3.6 Leuconostoc mesenteroides

Synonyms

EC Number Synonyms Comment Organism
4.1.3.6 More can be dissociated into components, two of which are identical with ec 2.8.3.10 and ec 4.1.3. 34 Leuconostoc mesenteroides
4.1.3.34 More EC 4.1.3.6 can be dissociated into components, two of which are identical with EC 2.8.3.10 and EC 4.1.3.34 Leuconostoc mesenteroides