EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.3.3 | Cu2+ | - |
Escherichia coli | |
4.1.3.3 | Hg2+ | - |
Escherichia coli | |
4.1.3.3 | Mn2+ | 20% residual activity at 10 mM | Escherichia coli | |
4.1.3.3 | N-ethylmaleimide | 25-27% residual activity at 1 mM | Escherichia coli | |
4.1.3.3 | p-chloromercuribenzoate | 2-7% residual activity at 1 mM | Escherichia coli | |
4.1.3.3 | Zn2+ | 1% residual activity at 10 mM | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.3.3 | 4.6 | - |
N-glycolylneuraminic acid | - |
Escherichia coli | |
4.1.3.3 | 4.8 | - |
N-acetylneuraminic acid | - |
Escherichia coli | |
4.1.3.3 | 7.7 | - |
N-acetyl-D-mannosamine | - |
Escherichia coli | |
4.1.3.3 | 8.3 | - |
pyruvate | - |
Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | 135000 | - |
gel filtration | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.3 | N-Acetylmannosamine + pyruvate | Escherichia coli | N-acetylneuraminic acid is intermediate for colomic acid biosynthesis | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.3.3 | Escherichia coli | - |
colominic acid producing strain | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.3.3 | - |
Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | 0.015 | - |
N-acetyl-D-mannosamine + pyruvate, 37°C | Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
4.1.3.3 | -20°C, 50 mM Tris/HCl, pH 8, 25 mM MgCl2, 1 mM PMSF, 20% v/v glycerol, stable under prolonged storage | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.3 | N-Acetyl-D-mannosamine + pyruvate | - |
Escherichia coli | N-Acetylneuraminic acid | - |
r | |
4.1.3.3 | N-Acetylmannosamine + pyruvate | N-acetylneuraminic acid is intermediate for colomic acid biosynthesis | Escherichia coli | ? | - |
? | |
4.1.3.3 | N-Acetylneuraminic acid | - |
Escherichia coli | N-Acetyl-D-mannosamine + pyruvate | - |
r | |
4.1.3.3 | N-Glycolylneuraminic acid | - |
Escherichia coli | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.3.3 | tetramer | 4*33000, SDS-PAGE | Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | 50 | - |
- |
Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | 37 | - |
in 50 mM Tris/HCl, pH 8, 25 mM MgCl2, 1 mM PMSF stable for at least 24 h | Escherichia coli |
4.1.3.3 | 50 | - |
stable for at least 40 min | Escherichia coli |
4.1.3.3 | 80 | - |
40% loss of activity after 10 min | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | 7.8 | 8 | elimination and condensation | Escherichia coli |