Literature summary extracted from

Comb, D.G.; Roseman, S.
The sialic acids. I. Structure and enzymatic synthesis of N-acetylneuraminic acid (1960), J. Biol. Chem., 235, 2529-2537.

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.1.3.3	3.3	-	N-acetylneuraminic acid	-	Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4.1.3.3	N-Acetylmannosamine + pyruvate	Escherichia coli	-	?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.1.3.3	Escherichia coli	-	-	-
4.1.3.3	Rattus norvegicus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.1.3.3	-	Escherichia coli

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
4.1.3.3	brain	-	Rattus norvegicus	-
4.1.3.3	liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.1.3.3	10	-	N-acetylneuraminic acid	Escherichia coli

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.1.3.3	N-Acetyl-D-mannosamine + pyruvate	-	Escherichia coli	N-Acetylneuraminic acid	-	r
4.1.3.3	N-Acetyl-D-mannosamine + pyruvate	-	Rattus norvegicus	N-Acetylneuraminic acid	-	r
4.1.3.3	N-Acetylmannosamine + pyruvate	-	Escherichia coli	?	-	?
4.1.3.3	N-Acetylneuraminic acid	-	Escherichia coli	N-Acetyl-D-mannosamine + pyruvate	-	r
4.1.3.3	N-Acetylneuraminic acid	-	Rattus norvegicus	N-Acetyl-D-mannosamine + pyruvate	-	r
4.1.3.3	N-Glycolylneuraminic acid	-	Escherichia coli	?	-	?

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Release 2023.1 (January 2023)