Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Uchida, Y.; Tsukada, Y.; Sugimori, T.
    Purification and properties of N-acetylneuraminate lyase from Escherichia coli (1984), J. Biochem., 96, 507-522.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.3.3 analysis enzymatic determination of sialic acid Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.3 Ag+ 0% residual activity at 0.1 mM, 6% residual activity at 0.01 mM Escherichia coli
4.1.3.3 Cu2+ 37% residual activity at 1 mM, 89% residual activity at 0.1 mM Escherichia coli
4.1.3.3 Hg2+ 0% residual activity at 0.1 mM, 22% residual activity at 0.01 mM Escherichia coli
4.1.3.3 hydrogen peroxide 0% residual activity at 10 mM, 16% residual activity at 1 mM Escherichia coli
4.1.3.3 Iodine 0% residual activity at 0.01 mM, 45% residual activity at 0.001 mM Escherichia coli
4.1.3.3 N-bromosuccinimide 0% residual activity at 0.01 mM, 36% residual activity at 0.001 mM Escherichia coli
4.1.3.3 p-chloromercuribenzoate 0% residual activity at 0.1 mM, 9% residual activity at 0.01 mM Escherichia coli
4.1.3.3 pyruvate elimination Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.3 3.6
-
N-acetylneuraminic acid
-
Escherichia coli
4.1.3.3 4.3
-
N-glycolylneuraminic acid
-
Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.3 additional information no requirement Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.3 33000
-
3 * 33000, SDS-PAGE Escherichia coli
4.1.3.3 98000
-
gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.3 Escherichia coli
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.1.3.3 sensitive to N-bromosuccinimide, H2O2 and iodine Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.3
-
Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.3 additional information
-
-
Escherichia coli
4.1.3.3 56.8
-
N-acetylneuraminic acid Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.3 additional information condensation: N-acetylglucosamine, N-acetylgalactosamine, glucosamine and galactosamine cannot replace N-acetylmannosamine. Phosphoenolpyruvate, lactate, beta-hydroxypyruvate and other pyruvate derivatives cannot replace pyruvate Escherichia coli ?
-
?
4.1.3.3 N-Acetyl-D-mannosamine + pyruvate
-
Escherichia coli N-Acetylneuraminic acid
-
r
4.1.3.3 N-Acetylneuraminic acid
-
Escherichia coli N-Acetyl-D-mannosamine + pyruvate
-
r
4.1.3.3 N-Glycolylneuraminic acid
-
Escherichia coli ?
-
?

Subunits

EC Number Subunits Comment Organism
4.1.3.3 trimer 3 * 33000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.3 75
-
-
Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.3.3 20 75
-
Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.3 additional information
-
pyruvate protects against heat inactivation Escherichia coli
4.1.3.3 60
-
stable up to Escherichia coli
4.1.3.3 80
-
77% loss of activity after 10 min, in presence of 5 mM pyruvate 8% loss of activity after 10 min Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.3 7.7
-
elimination and condensation Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.3.3 5 9
-
Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.3.3 6 9 at 37°C stable for 24 h Escherichia coli