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Literature summary extracted from

  • Hertel, S.C.; Hieke, M.; Gröger, D.
    Anthranilate synthase from Ruta graveolens: partial purification and properties (1991), Biochem. Physiol. Pflanz., 187, 121-129.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.27 Ba2+
-
 Ruta graveolens
4.1.3.27 Co2+ 10 mM, 67% inhibition of enzyme from cell line R-15, 33% inhibition of enzyme from cell line R-20 Ruta graveolens
4.1.3.27 Cu2+
-
 Ruta graveolens
4.1.3.27 Hg2+ 1 mM HgCl2, 94.1% inhibition Ruta graveolens
4.1.3.27 L-Trp competitive with respect to chorismate Ruta graveolens
4.1.3.27 MnCl2
-
 Ruta graveolens
4.1.3.27 Pb2+
-
 Ruta graveolens
4.1.3.27 PCMB 1 mM, 88.6% inhibition Ruta graveolens
4.1.3.27 Zn2+
-
 Ruta graveolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.27 0.18
-
 Gln cell line R-15 Ruta graveolens
4.1.3.27 0.22
-
 chorismate cell line R-20 Ruta graveolens
4.1.3.27 0.25
-
 Gln cell line R-20 Ruta graveolens
4.1.3.27 0.28
-
 chorismate cell line R-15 Ruta graveolens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.27 Co2+ at 1-5 mM Ruta graveolens
4.1.3.27 Fe2+ 1 mM, can partially replace Mg2+ in activation Ruta graveolens
4.1.3.27 Mg2+
-
 Ruta graveolens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.27 220000
-
 gel filtration Ruta graveolens

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.27 Ruta graveolens
-
 a high rutacridone producing cell line R-20 and low rutacrinone producing cell line R-15
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.27 partial, from a high rutacridone producing cell line R-20 and from a low rutacrinone producing cell line R-15 Ruta graveolens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.27 additional information
-
-
 Ruta graveolens

Storage Stability

EC Number Storage Stability Organism
4.1.3.27 -20°C, unstable Ruta graveolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.27 chorismate + L-Gln
-
 Ruta graveolens anthranilate + pyruvate + L-glutamate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.27 37 40
-
 Ruta graveolens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.3.27 25 45 25°C: about 70% of maximal activity, 45°C: about 85% of maximal activity Ruta graveolens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.27 35
-
 5 min, about 25% loss of activity Ruta graveolens
4.1.3.27 40
-
 5 min, about 50% loss of activity Ruta graveolens
4.1.3.27 45
-
 5 min, about 80% loss of activity Ruta graveolens
4.1.3.27 50
-
 5 min, about 90% loss of activity Ruta graveolens