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Literature summary extracted from

  • Poulsen, C.; Bongaerts, R.J.M.; Verpoorte, R.
    Purification and characterization of anthranilate synthase from Catharanthus roseus (1993), Eur. J. Biochem., 212, 431-440.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.27 L-Trp
-
 Catharanthus roseus
4.1.3.27 tryptamine
-
 Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.27 0.067
-
 chorismate
-
 Catharanthus roseus
4.1.3.27 0.37
-
 Gln
-
 Catharanthus roseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.3.27 protoplast
-
 Catharanthus roseus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.27 Mg2+ Km for MgCl2: 0.26 mM Catharanthus roseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.27 25500
-
 2 * 25500 + 2 * 67000, SDS-PAGE Catharanthus roseus
4.1.3.27 67000
-
 2 * 25500 + 2 * 67000, SDS-PAGE Catharanthus roseus
4.1.3.27 143000
-
 gel filtration Catharanthus roseus

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.27 Catharanthus roseus
-
 monofunctional enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.27
-
 Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.27 chorismate + L-Gln
-
 Catharanthus roseus anthranilate + pyruvate + L-glutamate
-
 ?
4.1.3.27 chorismate + NH4+
-
 Catharanthus roseus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.27 tetramer 2 * 25500 + 2 * 67000, SDS-PAGE Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.27 7.5 8.3 Gln-dependent activity Catharanthus roseus