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Literature summary extracted from

  • Francis, M.M.; Vining, L.C.; Westlake, D.W.S.
    Characterization and regulation of anthranilate synthetase from a chloramphenicol-producing streptomycete (1978), J. Bacteriol., 134, 10-16.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.27 anthranilate
-
 Streptomyces sp.
4.1.3.27 L-Trp
-
 Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.27 28000
-
 1 * 28000 + 1 * 72000, gel filtration after separation of subunits Streptomyces sp.
4.1.3.27 72000
-
 1 * 28000 + 1 * 72000, gel filtration after separation of subunits Streptomyces sp.
4.1.3.27 94000 117000 gel filtration Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.27 additional information Streptomyces sp. synthesis of the enzyme is repressed by His, anthranilate, Trp and p-aminobenzoate ?
-
 ?
4.1.3.27 additional information Streptomyces sp. 3022a synthesis of the enzyme is repressed by His, anthranilate, Trp and p-aminobenzoate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.27 Streptomyces sp.
-
-
-
4.1.3.27 Streptomyces sp. 3022a
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.27 additional information
-
-
 Streptomyces sp.

Storage Stability

EC Number Storage Stability Organism
4.1.3.27 -20°C, activity of partially purified enzyme declines during storage Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.27 chorismate + L-Gln
-
 Streptomyces sp. anthranilate + pyruvate + L-glutamate
-
 ?
4.1.3.27 chorismate + L-Gln
-
 Streptomyces sp. 3022a anthranilate + pyruvate + L-glutamate
-
 ?
4.1.3.27 chorismate + NH4+
-
 Streptomyces sp. ?
-
 ?
4.1.3.27 chorismate + NH4+
-
 Streptomyces sp. 3022a ?
-
 ?
4.1.3.27 additional information the 72000 Da subunit converts chorismate to anthranilate using NH4+. The 28000 Da subunit confers the enzyme the ability to use Gln instead of NH4+ as substrate Streptomyces sp. ?
-
 ?
4.1.3.27 additional information synthesis of the enzyme is repressed by His, anthranilate, Trp and p-aminobenzoate Streptomyces sp. ?
-
 ?
4.1.3.27 additional information the 72000 Da subunit converts chorismate to anthranilate using NH4+. The 28000 Da subunit confers the enzyme the ability to use Gln instead of NH4+ as substrate Streptomyces sp. 3022a ?
-
 ?
4.1.3.27 additional information synthesis of the enzyme is repressed by His, anthranilate, Trp and p-aminobenzoate Streptomyces sp. 3022a ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.27 dimer 1 * 28000 + 1 * 72000, gel filtration after separation of subunits Streptomyces sp.
4.1.3.27 More the 72000 Da subunit converts chorismate to anthranilate using NH4+. The 28000 Da subunit confers the enzyme the ability to use Gln instead of NH4+ as substrate Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.27 additional information
-
 NH4+-dependent activity increases linearly through pH 9.0 without reaching a maximum Streptomyces sp.
4.1.3.27 7.2 7.6 Gln-dependent activity Streptomyces sp.