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Literature summary extracted from

  • Bode, R.; Birnbaum, D.
    Die Enzyme der Biosynthese aromatischer Aminosäuren bei Hansenula henricii: Charakterisierung der Anthranilat-synthase (1978), Biochem. Physiol. Pflanz., 172, 245-253.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.27 0.023
-
 chorismate Gln-dependent enzyme that is composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits (I2II2) Ogataea henricii
4.1.3.27 0.094
-
 Gln Gln-dependent enzyme that is composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits (I2II2) Ogataea henricii
4.1.3.27 1.2
-
 NH4+ NH4+-dependent enzyme, component I Ogataea henricii
4.1.3.27 3.1
-
 chorismate NH4+-dependent enzyme, component I Ogataea henricii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.27 Mg2+ required Ogataea henricii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.27 20000
-
 2 * 20000 + 2 * 50000, Gln-dependent enzyme, composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits, I2II2, gel filtration Ogataea henricii
4.1.3.27 50000
-
 NH4-dependent enzyme, i.e. component I, gel filtration Ogataea henricii
4.1.3.27 160000
-
 Gln-dependent enzyme composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits (I2II2), gel filtration Ogataea henricii

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.27 Ogataea henricii
-
 CCY 38-10-2, the organism contains an NH4-dependent enzyme (component I) and a Gln-dependent enzyme that is composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits (I2II2)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.27
-
 Ogataea henricii

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.3.27 chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate sequential mechanism Ogataea henricii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.27 43
-
-
 Ogataea henricii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.27 chorismate + L-Gln
-
 Ogataea henricii anthranilate + pyruvate + L-glutamate
-
 ?
4.1.3.27 chorismate + NH4+
-
 Ogataea henricii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.27 More the organism contains an NH4-dependent enzyme, i.e. component I, and a Gln-dependent enzyme that is composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits, i.e. I2II2 Ogataea henricii
4.1.3.27 tetramer 2 * 20000 + 2 * 50000, Gln-dependent enzyme, composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits, I2II2, gel filtration Ogataea henricii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.27 6.7
-
 Gln-dependent reaction Ogataea henricii
4.1.3.27 7.3
-
 NH4+-dependent enzyme Ogataea henricii