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Literature summary extracted from

  • Friedrich, C.G.; Friedrich, B.; Schlegel, H.G.
    Aromatic amino acid biosynthesis in Alcaligenes eutrophus H 16 III. Properites and regulation of anthranilate synthase (1976), Arch. Microbiol., 107, 125-131.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.27 L-Trp noncompetitive with respect to L-Gln, inhibitory effect increases with rising pH Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.27 0.005
-
 chorismate
-
 Cupriavidus necator
4.1.3.27 0.56
-
 Gln
-
 Cupriavidus necator

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.27 Mg2+ required Cupriavidus necator
4.1.3.27 Mg2+ maximal activity at 0.03 mM Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.27 126000
-
 sucrose density gradient centrifugation Cupriavidus necator
4.1.3.27 158000
-
 gel filtration Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.27 Cupriavidus necator
-
 H 16
-
4.1.3.27 Cupriavidus necator H 16
-
 H 16
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.27 partial Cupriavidus necator

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.27 additional information
-
-
 Cupriavidus necator

Storage Stability

EC Number Storage Stability Organism
4.1.3.27 4°C, TEA-sulfuric acid buffer, PH 7.2, stable for at least 2 days Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.27 chorismate + L-Gln
-
 Cupriavidus necator anthranilate + pyruvate + L-glutamate
-
 ?
4.1.3.27 chorismate + L-Gln
-
 Cupriavidus necator H 16 anthranilate + pyruvate + L-glutamate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.27 50
-
-
 Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.27 8.7
-
 Gln-dependent activity Cupriavidus necator