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Literature summary extracted from

  • Queener, S.W.; Queener, S.F.; Meeks, J.R.; Gunsalus, I.C.
    Anthranilate synthase from Pseudomonas putida. Purification and properties of a two-component enzyme (1973), J. Biol. Chem., 248, 151-161.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.27 6-diazo-5-oxo-L-norleucine
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.27 0.007
-
 chorismate enzyme component ASI, with Gln as cosubstrate Pseudomonas putida
4.1.3.27 0.09
-
 chorismate two-component enzyme, ASI,ASII, with ammonium as cosubstrate Pseudomonas putida
4.1.3.27 0.6
-
 Gln
-
 Pseudomonas putida
4.1.3.27 26
-
 NH4+ two-component enzyme, ASI,ASII Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.27 18000
-
 1 * 18000 + 1 * 63400, sucrose density gradient centrifugation Pseudomonas putida
4.1.3.27 63400
-
 1 * 18000 + 1 * 63400, sucrose density gradient centrifugation Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.27 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.27
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.27 additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.27 chorismate + L-Gln
-
 Pseudomonas putida anthranilate + pyruvate + L-glutamate
-
 ?
4.1.3.27 chorismate + NH4+ reaction is catalyzed by component I alone and in combination with component II Pseudomonas putida ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.27 dimer 1 * 18000 + 1 * 63400, sucrose density gradient centrifugation Pseudomonas putida
4.1.3.27 More glutamine, 10 mM, enhances binding of AS I and II, as does Mg2+. ASI and ASII play a joint role in the binding of L-Gln, negligible binding to either component alone Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.27 8.8
-
 reaction of ASI with chorismate and NH4+ Pseudomonas putida