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Literature summary extracted from

  • Dimroth, P.; Buckel, W.; Loyal, R.; Eggerer, H.
    Isolation and function of the subunits of citramalate lyase and formation of hybrids with the subunits of citrate lyase (1977), Eur. J. Biochem., 80, 469-477.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.25 Mg2+ required Clostridium tetanomorphum
4.1.3.25 Mg2+ the isolated beta-subunit of citramalate lyase becomes saturated at 10 mM Mg2+ Clostridium tetanomorphum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.11 acetyl-[acyl-carrier protein] + citramalate Clostridium tetanomorphum biological significant reaction, alpha-subunit of citramalate lyase enzyme complex citramalyl-[acyl-carrier protein] + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.11 Clostridium tetanomorphum
-
 alpha-subunit of the citramalate enzyme complex
-
4.1.3.22 Clostridium tetanomorphum
-
-
-
4.1.3.25 Clostridium tetanomorphum
-
 EC 4.1.3.25 is the beta-subunit of EC 4.1.3.22
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.11
-
 Clostridium tetanomorphum

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.8.3.11 reconstitution of the enzyme complex at pH 8.0, 25°C Clostridium tetanomorphum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.11 additional information
-
-
 Clostridium tetanomorphum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.11 acetyl-CoA + citramalate alpha-subunit of the citramalate enzyme complex Clostridium tetanomorphum acetate + (3S)-citramalyl-CoA
-
 r
2.8.3.11 acetyl-[acyl-carrier protein] + citramalate substrate is the acetylated acyl carrier protein of citrate lyase or citramalate lyase enzyme complex Clostridium tetanomorphum citramalyl-[acyl-carrier protein] + acetate
-
 ?
2.8.3.11 acetyl-[acyl-carrier protein] + citramalate biological significant reaction, alpha-subunit of citramalate lyase enzyme complex Clostridium tetanomorphum citramalyl-[acyl-carrier protein] + acetate
-
 ?
2.8.3.11 additional information no substrate: citrate Clostridium tetanomorphum ?
-
 ?
2.8.3.11 additional information also catalyses citramalate dependent exchange reaction between acetate and acetyl-CoA Clostridium tetanomorphum ?
-
 ?
4.1.3.22 citramalate the alpha-subunit catalyzes the formation of acetyl-CoA and citramalate. The beta-subunit catalyzes the cleavage of (3S)-citramalyl-thio-acyl carrier protein lyase Clostridium tetanomorphum acetate + pyruvate
-
 ?
4.1.3.25 (3S)-citramalyl-thio-acylcarrier protein
-
 Clostridium tetanomorphum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.22 oligomer the alpha-subunit represents an acetyl-thio-acyl carrier protein:citramalate acyl carrier protein transferase and the beta-subunit a (3S)-citramalyl-thio-acyl carrier protein lyase Clostridium tetanomorphum
4.1.3.25 More EC 4.1.3.25 is the beta-subunit of EC 4.1.3.22 Clostridium tetanomorphum

Synonyms

EC Number Synonyms Comment Organism
4.1.3.25 More EC 4.1.3.25 is the beta-subunit of EC 4.1.3.22 Clostridium tetanomorphum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.3.11 25
-
 assay at Clostridium tetanomorphum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.3.11 8
-
 assay at Clostridium tetanomorphum