EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.1.3.22 | additional information | the enzyme contains acetyl thioester residues which play the central role in the catalytic mechanism | Clostridium tetanomorphum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.3.22 | iodoacetate | - |
Clostridium tetanomorphum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.3.22 | 520000 | 580000 | gel filtration, sucrose density gradient centrifugation | Clostridium tetanomorphum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.3.22 | Clostridium tetanomorphum | - |
- |
- |
4.1.3.25 | Clostridium tetanomorphum | - |
EC 4.1.3.25 is the beta-subunit of EC 4.1.3.22 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.3.22 | - |
Clostridium tetanomorphum |
4.1.3.25 | - |
Clostridium tetanomorphum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.3.22 | additional information | - |
- |
Clostridium tetanomorphum |
EC Number | Storage Stability | Organism |
---|---|---|
4.1.3.22 | -20°C, enzyme concentration 10 mg/ml, 0.1 M phoshate, pH 7.4, 1.0 mM MgCl2, stable for at least 6 months | Clostridium tetanomorphum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.22 | acetate + pyruvate | - |
Clostridium tetanomorphum | (+)-citramalate | - |
? | |
4.1.3.22 | citramalate | (+)-citramalate | Clostridium tetanomorphum | acetate + pyruvate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.3.22 | oligomer | 6 * 53000-56000, alpha, + 6 * 33000-36000, beta, + 6 * 10000-12000, gamma, SDS-PAGE | Clostridium tetanomorphum |
4.1.3.25 | ? | MW of beta-subunit is 33000-36000 Da, EC 4.1.3.25 is the beta-subunit of the enzyme complex EC 4.1.3.22, that has the structure(alpha,beta,gamma)6, SDS-PAGE | Clostridium tetanomorphum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.3.25 | More | EC 4.1.3.25 is the beta-subunit of EC 4.1.3.22 | Clostridium tetanomorphum |