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Literature summary extracted from
- Purification of bisphosphoglyceromutase, 2,3-bisphosphoglycerate phosphatase and phosphoglyceromutase from human erthrocytes. Three enzyme activities in one protein (1975), Eur. J. Biochem., 50, 581-593.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.4 | 2-Phosphoglycolate | - |
Homo sapiens |  |
| 5.4.2.4 | NaHSO3 | - |
Homo sapiens | |
| 5.4.2.4 | potassium phosphate | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.4 | 0.0094 | - |
2,3-diphosphoglycerate | peak II enzyme | Homo sapiens | |
| 5.4.2.4 | 0.0096 | - |
2,3-diphosphoglycerate | peak I enzyme | Homo sapiens | |
| 5.4.2.4 | 0.0222 | - |
2,3-diphosphoglycerate | peak III enzyme | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.4 | 29000 | - |
2 * 29000, SDS-PAGE | Homo sapiens |
| 5.4.2.4 | 60000 | - |
gel filtration | Homo sapiens |
| 5.4.2.4 | 63000 | - |
sedimentation equilibrium centrifugation | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.2.4 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.2.4 | copurification of EC 5.4.2.1 (phosphoglycerate mutase) and EC 3.1.3.13 (bisphosphoglycerate phosphatase), three activities in one enzyme protein | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.4.2.4 | erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.4 | 9.78 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.4 | 1,3-Diphosphoglycerate | - |
Homo sapiens | 2,3-Diphosphoglycerate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.2.4 | dimer | 2 * 29000, SDS-PAGE | Homo sapiens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.4 | 45 | - |
peak II enzyme stable, peak I and peak III enzyme 46% and 80% loss of activity in 10 min, respectively | Homo sapiens |
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