BRENDA - Enzyme Database

Purification and characterization of isocitrate lyase from Rhodopseudomonas sp. No. 7

Tahama, H.; Shinoyama, H.; Ando, A.; Fujh, T.; Agric. Biol. Chem. 54, 3177-3183 (1990)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
HgCl2
30% inhibition at 0.1 mM
Rhodopseudomonas sp.
4.1.3.1
iodoacetate
70% inhibition at 10 mM
Rhodopseudomonas sp.
4.1.3.1
malate
-
Rhodopseudomonas sp.
4.1.3.1
N-ethylmaleimide
40% inhibition at 1 mM
Rhodopseudomonas sp.
4.1.3.1
p-chloromercuribenzoate
70% inhibition at 0.1 mM
Rhodopseudomonas sp.
4.1.3.1
succinate
-
Rhodopseudomonas sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.136
-
DL-isocitrate
-
Rhodopseudomonas sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Ba2+
Ba2+ less effective activator than Mg2+
Rhodopseudomonas sp.
4.1.3.1
Mg2+
non-essential activator
Rhodopseudomonas sp.
4.1.3.1
Mn2+
Mn2+ less effective activator than Mg2+
Rhodopseudomonas sp.
4.1.3.1
Ni2+
less efficient activator than Mg2+
Rhodopseudomonas sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
62000
-
4 * 62000, SDS-PAGE
Rhodopseudomonas sp.
4.1.3.1
250000
-
gel filtration
Rhodopseudomonas sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Rhodopseudomonas sp.
glyoxylate cycle
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Rhodopseudomonas sp.
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Rhodopseudomonas sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
8.73
-
-
Rhodopseudomonas sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33155
Rhodopseudomonas sp.
succinate + glyoxylate
-
33155
Rhodopseudomonas sp.
-
4.1.3.1
isocitrate
glyoxylate cycle
33155
Rhodopseudomonas sp.
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 62000, SDS-PAGE
Rhodopseudomonas sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.3.1
45
-
-
Rhodopseudomonas sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
50
-
low stability at temperatures above
Rhodopseudomonas sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.5
-
-
Rhodopseudomonas sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.1.3.1
6
7
stable
Rhodopseudomonas sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
HgCl2
30% inhibition at 0.1 mM
Rhodopseudomonas sp.
4.1.3.1
iodoacetate
70% inhibition at 10 mM
Rhodopseudomonas sp.
4.1.3.1
malate
-
Rhodopseudomonas sp.
4.1.3.1
N-ethylmaleimide
40% inhibition at 1 mM
Rhodopseudomonas sp.
4.1.3.1
p-chloromercuribenzoate
70% inhibition at 0.1 mM
Rhodopseudomonas sp.
4.1.3.1
succinate
-
Rhodopseudomonas sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.136
-
DL-isocitrate
-
Rhodopseudomonas sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Ba2+
Ba2+ less effective activator than Mg2+
Rhodopseudomonas sp.
4.1.3.1
Mg2+
non-essential activator
Rhodopseudomonas sp.
4.1.3.1
Mn2+
Mn2+ less effective activator than Mg2+
Rhodopseudomonas sp.
4.1.3.1
Ni2+
less efficient activator than Mg2+
Rhodopseudomonas sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
62000
-
4 * 62000, SDS-PAGE
Rhodopseudomonas sp.
4.1.3.1
250000
-
gel filtration
Rhodopseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Rhodopseudomonas sp.
glyoxylate cycle
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Rhodopseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
8.73
-
-
Rhodopseudomonas sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33155
Rhodopseudomonas sp.
succinate + glyoxylate
-
33155
Rhodopseudomonas sp.
-
4.1.3.1
isocitrate
glyoxylate cycle
33155
Rhodopseudomonas sp.
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 62000, SDS-PAGE
Rhodopseudomonas sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.3.1
45
-
-
Rhodopseudomonas sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
50
-
low stability at temperatures above
Rhodopseudomonas sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.5
-
-
Rhodopseudomonas sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.1.3.1
6
7
stable
Rhodopseudomonas sp.