BRENDA - Enzyme Database

Purification and properties of isocitrate lyase from Candida brassicae E-17

Nakamura, K.; Amano, Y.; Nakadate, M.; Kagami, M.; J. Ferment. Bioeng. 67, 153-157 (1989)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
2-mercaptoethanol
increase of activity
Candida brassicae
4.1.3.1
cysteine
increase of activity
Candida brassicae
4.1.3.1
dithioerythritol
increase of activity
Candida brassicae
4.1.3.1
dithiothreitol
increase of activity
Candida brassicae
4.1.3.1
glutathione
increase of activity
Candida brassicae
4.1.3.1
thiol compounds
increase of activity
Candida brassicae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
2-mercaptoethanol
increase of activity
Candida brassicae
4.1.3.1
glutathione
increase of activity
Candida brassicae
4.1.3.1
glycolate
-
Candida brassicae
4.1.3.1
malonate
-
Candida brassicae
4.1.3.1
Mn2+
inhibition in presence of Mg2+, activation in absence of Mg2+
Candida brassicae
4.1.3.1
succinate
-
Candida brassicae
4.1.3.1
Zn2+
-
Candida brassicae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.62
-
DL-isocitrate
imidazole-HCl buffer pH 6.85
Candida brassicae
4.1.3.1
1.532
-
DL-isocitrate
sodium phosphate buffer, pH 6.85
Candida brassicae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Ba2+
Ba2+ less effective activator than Mg2+
Candida brassicae
4.1.3.1
Mg2+
maximal activity at 8 mM
Candida brassicae
4.1.3.1
Mn2+
Mn2+ less effective activator than Mg2+
Candida brassicae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
290000
-
gel filtration
Candida brassicae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Candida brassicae
glyoxylate cycle
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Candida brassicae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Candida brassicae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.1.3.1
8.01
-
-
Candida brassicae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33153
Candida brassicae
succinate + glyoxylate
-
33153
Candida brassicae
-
4.1.3.1
isocitrate
glyoxylate cycle
33153
Candida brassicae
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4*71000, SDS-PAGE
Candida brassicae
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
4.1.3.1
37
-
-
Candida brassicae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.8
-
-
Candida brassicae
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.1.3.1
6
7
stable
Candida brassicae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
2-mercaptoethanol
increase of activity
Candida brassicae
4.1.3.1
cysteine
increase of activity
Candida brassicae
4.1.3.1
dithioerythritol
increase of activity
Candida brassicae
4.1.3.1
dithiothreitol
increase of activity
Candida brassicae
4.1.3.1
glutathione
increase of activity
Candida brassicae
4.1.3.1
thiol compounds
increase of activity
Candida brassicae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
2-mercaptoethanol
increase of activity
Candida brassicae
4.1.3.1
glutathione
increase of activity
Candida brassicae
4.1.3.1
glycolate
-
Candida brassicae
4.1.3.1
malonate
-
Candida brassicae
4.1.3.1
Mn2+
inhibition in presence of Mg2+, activation in absence of Mg2+
Candida brassicae
4.1.3.1
succinate
-
Candida brassicae
4.1.3.1
Zn2+
-
Candida brassicae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.62
-
DL-isocitrate
imidazole-HCl buffer pH 6.85
Candida brassicae
4.1.3.1
1.532
-
DL-isocitrate
sodium phosphate buffer, pH 6.85
Candida brassicae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Ba2+
Ba2+ less effective activator than Mg2+
Candida brassicae
4.1.3.1
Mg2+
maximal activity at 8 mM
Candida brassicae
4.1.3.1
Mn2+
Mn2+ less effective activator than Mg2+
Candida brassicae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
290000
-
gel filtration
Candida brassicae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Candida brassicae
glyoxylate cycle
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Candida brassicae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.1.3.1
8.01
-
-
Candida brassicae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33153
Candida brassicae
succinate + glyoxylate
-
33153
Candida brassicae
-
4.1.3.1
isocitrate
glyoxylate cycle
33153
Candida brassicae
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4*71000, SDS-PAGE
Candida brassicae
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
4.1.3.1
37
-
-
Candida brassicae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.8
-
-
Candida brassicae
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.1.3.1
6
7
stable
Candida brassicae