BRENDA - Enzyme Database

Soybean isocitrate lyase: Purification, immunoassay and N-terminal sequence

Polanowski, A.J.; Obendorf, R.L.; Plant Physiol. Biochem. 29, 119-129 (1991)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.093
-
DL-isocitrate
-
Glycine max
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
63000
-
2 * 63000, calculation from amino acid sequence
Glycine max
4.1.3.1
64000
-
2 * 64000, SDS-PAGE
Glycine max
4.1.3.1
130000
-
gel filtration
Glycine max
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Glycine max
glyoxylate cycle
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Glycine max
-
soybean
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Glycine max
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
cotyledon
-
Glycine max
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
9.48
-
-
Glycine max
Storage Stability
EC Number
Storage Stability
Organism
4.1.3.1
-12°C, 20% glycerol, 21 days, 20% loss of activity
Glycine max
4.1.3.1
-12°C, 20% glycerol, 7 days, 20% loss of activity
Glycine max
4.1.3.1
-12°C, without glycerol, 4 h, 100% loss of activity
Glycine max
4.1.3.1
4°C, 20% glycerol, 7 days, 30% loss of activity
Glycine max
4.1.3.1
4°C, without glycerol, 7 days, 50% loss of activity
Glycine max
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33152
Glycine max
succinate + glyoxylate
-
33152
Glycine max
-
4.1.3.1
isocitrate
glyoxylate cycle
33152
Glycine max
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
dimer
2 * 63000, calculation from amino acid sequence; 2 * 64000, SDS-PAGE
Glycine max
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.3.1
50
-
-
Glycine max
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
50
-
heating to temperatures above caused loss of activity
Glycine max
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
8
-
-
Glycine max
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.3.1
7
9
30% of optimal activity at pH 7 and pH 9
Glycine max
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.093
-
DL-isocitrate
-
Glycine max
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
63000
-
2 * 63000, calculation from amino acid sequence
Glycine max
4.1.3.1
64000
-
2 * 64000, SDS-PAGE
Glycine max
4.1.3.1
130000
-
gel filtration
Glycine max
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Glycine max
glyoxylate cycle
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Glycine max
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
cotyledon
-
Glycine max
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
9.48
-
-
Glycine max
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.3.1
-12°C, 20% glycerol, 21 days, 20% loss of activity
Glycine max
4.1.3.1
-12°C, 20% glycerol, 7 days, 20% loss of activity
Glycine max
4.1.3.1
-12°C, without glycerol, 4 h, 100% loss of activity
Glycine max
4.1.3.1
4°C, 20% glycerol, 7 days, 30% loss of activity
Glycine max
4.1.3.1
4°C, without glycerol, 7 days, 50% loss of activity
Glycine max
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33152
Glycine max
succinate + glyoxylate
-
33152
Glycine max
-
4.1.3.1
isocitrate
glyoxylate cycle
33152
Glycine max
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
dimer
2 * 63000, calculation from amino acid sequence; 2 * 64000, SDS-PAGE
Glycine max
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.3.1
50
-
-
Glycine max
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
50
-
heating to temperatures above caused loss of activity
Glycine max
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
8
-
-
Glycine max
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.3.1
7
9
30% of optimal activity at pH 7 and pH 9
Glycine max