BRENDA - Enzyme Database

Characterization of isocitrate lyase from the yeast Yarrowia lipolytica

Hönes, I.; Simon, M.; Weber, H.; J. Basic Microbiol. 31, 251-258 (1991)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
methylenesuccinate
-
Yarrowia lipolytica
4.1.3.1
oxalacetate
-
Yarrowia lipolytica
4.1.3.1
phosphoenolpyruvate
-
Yarrowia lipolytica
4.1.3.1
succinate
-
Yarrowia lipolytica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.3
-
threo-Ds-isocitrate
-
Yarrowia lipolytica
4.1.3.1
0.6
-
DL-isocitrate
-
Yarrowia lipolytica
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
50000
-
4 * 50000, SDS-PAGE
Yarrowia lipolytica
4.1.3.1
200000
210000
gel filtration
Yarrowia lipolytica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Yarrowia lipolytica
glyoxylate cycle
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Yarrowia lipolytica
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Yarrowia lipolytica
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
7.4
-
-
Yarrowia lipolytica
Storage Stability
EC Number
Storage Stability
Organism
4.1.3.1
-20°C, phosphate buffer, pH 6.0, 3 mM MgCl2, 10% glycerol, two months, 8% loss of activity
Yarrowia lipolytica
4.1.3.1
-20°C, phosphate buffer, pH 6.0, 3 mM MgCl2, one week, 90% loss of activity
Yarrowia lipolytica
4.1.3.1
4°C, phosphate buffer, pH 6.0, 3 mM MgCl2, one week, 50% loss of activity
Yarrowia lipolytica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33149
Yarrowia lipolytica
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33149
Yarrowia lipolytica
succinate + glyoxylate
-
33149
Yarrowia lipolytica
-
4.1.3.1
isocitrate
glyoxylate cycle
33149
Yarrowia lipolytica
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 50000, SDS-PAGE
Yarrowia lipolytica
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
40
-
stable at temperatures up to 40°C, rapid decrease of activity at higher temperatures
Yarrowia lipolytica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6
-
-
Yarrowia lipolytica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
methylenesuccinate
-
Yarrowia lipolytica
4.1.3.1
oxalacetate
-
Yarrowia lipolytica
4.1.3.1
phosphoenolpyruvate
-
Yarrowia lipolytica
4.1.3.1
succinate
-
Yarrowia lipolytica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.3
-
threo-Ds-isocitrate
-
Yarrowia lipolytica
4.1.3.1
0.6
-
DL-isocitrate
-
Yarrowia lipolytica
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
50000
-
4 * 50000, SDS-PAGE
Yarrowia lipolytica
4.1.3.1
200000
210000
gel filtration
Yarrowia lipolytica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Yarrowia lipolytica
glyoxylate cycle
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Yarrowia lipolytica
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
7.4
-
-
Yarrowia lipolytica
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.3.1
-20°C, phosphate buffer, pH 6.0, 3 mM MgCl2, 10% glycerol, two months, 8% loss of activity
Yarrowia lipolytica
4.1.3.1
-20°C, phosphate buffer, pH 6.0, 3 mM MgCl2, one week, 90% loss of activity
Yarrowia lipolytica
4.1.3.1
4°C, phosphate buffer, pH 6.0, 3 mM MgCl2, one week, 50% loss of activity
Yarrowia lipolytica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33149
Yarrowia lipolytica
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33149
Yarrowia lipolytica
succinate + glyoxylate
-
33149
Yarrowia lipolytica
-
4.1.3.1
isocitrate
glyoxylate cycle
33149
Yarrowia lipolytica
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 50000, SDS-PAGE
Yarrowia lipolytica
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
40
-
stable at temperatures up to 40°C, rapid decrease of activity at higher temperatures
Yarrowia lipolytica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6
-
-
Yarrowia lipolytica