BRENDA - Enzyme Database

Characterization, gene cloning and expression of isocitrate lyase involved in the assimilation of one-carbon compounds in Hyphomicrobium methylovorum GM2

Tanaka, Y.; Yoshida, T.; Watanabe, K.; Izumi, Y.; Mitsunaga, T.; Eur. J. Biochem. 249, 820-825 (1997)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.3.1
expression in Escherichia coli
Hyphomicrobium methylovorum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
Ag+
-
Hyphomicrobium methylovorum
4.1.3.1
Al3+
-
Hyphomicrobium methylovorum
4.1.3.1
Cd2+
-
Hyphomicrobium methylovorum
4.1.3.1
DL-penicillamin
-
Hyphomicrobium methylovorum
4.1.3.1
glycolate
20% inhibition at 1 mM
Hyphomicrobium methylovorum
4.1.3.1
iodoacetamide
-
Hyphomicrobium methylovorum
4.1.3.1
KCl
-
Hyphomicrobium methylovorum
4.1.3.1
KCN
-
Hyphomicrobium methylovorum
4.1.3.1
Li+
-
Hyphomicrobium methylovorum
4.1.3.1
Mn2+
-
Hyphomicrobium methylovorum
4.1.3.1
NaCl
-
Hyphomicrobium methylovorum
4.1.3.1
oxalate
77% inhibition at 1 mM
Hyphomicrobium methylovorum
4.1.3.1
Sr2+
-
Hyphomicrobium methylovorum
4.1.3.1
succinate
38% inhibition at 1 mM
Hyphomicrobium methylovorum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.051
-
threo-Ds-isocitrate
-
Hyphomicrobium methylovorum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Ba2+
no activation
Hyphomicrobium methylovorum
4.1.3.1
Mg2+
increase of activity
Hyphomicrobium methylovorum
4.1.3.1
Mn2+
Mn2+ 27% as effective as Mg2+; no activity in absence of exogenous cation
Hyphomicrobium methylovorum
4.1.3.1
Sr2+
no activation
Hyphomicrobium methylovorum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
62000
-
4 * 62000, SDS-PAGE
Hyphomicrobium methylovorum
4.1.3.1
250000
-
gel filtration
Hyphomicrobium methylovorum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Hyphomicrobium methylovorum
assimilation of one-carbon compounds in the icl+ serine pathway
?
-
-
-
4.1.3.1
isocitrate
Hyphomicrobium methylovorum
glyoxylate cycle
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Hyphomicrobium methylovorum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Hyphomicrobium methylovorum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
2.4
-
-
Hyphomicrobium methylovorum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33148
Hyphomicrobium methylovorum
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33148
Hyphomicrobium methylovorum
succinate + glyoxylate
-
33148
Hyphomicrobium methylovorum
-
4.1.3.1
isocitrate
assimilation of one-carbon compounds in the icl+ serine pathway
33148
Hyphomicrobium methylovorum
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33148
Hyphomicrobium methylovorum
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 62000, SDS-PAGE
Hyphomicrobium methylovorum
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.3.1
45
-
-
Hyphomicrobium methylovorum
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
25
-
no loss of activity after 30 min
Hyphomicrobium methylovorum
4.1.3.1
30
-
16% loss of activity after 30 min
Hyphomicrobium methylovorum
4.1.3.1
40
-
30% loss of activity after 30 min
Hyphomicrobium methylovorum
4.1.3.1
50
-
82% loss of activity after 30 min
Hyphomicrobium methylovorum
4.1.3.1
60
-
100% loss of activity after 30 min
Hyphomicrobium methylovorum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.5
-
-
Hyphomicrobium methylovorum
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.1.3.1
7.5
9
at 15°C stable for 30 min
Hyphomicrobium methylovorum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
expression in Escherichia coli
Hyphomicrobium methylovorum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
Ag+
-
Hyphomicrobium methylovorum
4.1.3.1
Al3+
-
Hyphomicrobium methylovorum
4.1.3.1
Cd2+
-
Hyphomicrobium methylovorum
4.1.3.1
DL-penicillamin
-
Hyphomicrobium methylovorum
4.1.3.1
glycolate
20% inhibition at 1 mM
Hyphomicrobium methylovorum
4.1.3.1
iodoacetamide
-
Hyphomicrobium methylovorum
4.1.3.1
KCl
-
Hyphomicrobium methylovorum
4.1.3.1
KCN
-
Hyphomicrobium methylovorum
4.1.3.1
Li+
-
Hyphomicrobium methylovorum
4.1.3.1
Mn2+
-
Hyphomicrobium methylovorum
4.1.3.1
NaCl
-
Hyphomicrobium methylovorum
4.1.3.1
oxalate
77% inhibition at 1 mM
Hyphomicrobium methylovorum
4.1.3.1
Sr2+
-
Hyphomicrobium methylovorum
4.1.3.1
succinate
38% inhibition at 1 mM
Hyphomicrobium methylovorum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.051
-
threo-Ds-isocitrate
-
Hyphomicrobium methylovorum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Ba2+
no activation
Hyphomicrobium methylovorum
4.1.3.1
Mg2+
increase of activity
Hyphomicrobium methylovorum
4.1.3.1
Mn2+
Mn2+ 27% as effective as Mg2+; no activity in absence of exogenous cation
Hyphomicrobium methylovorum
4.1.3.1
Sr2+
no activation
Hyphomicrobium methylovorum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
62000
-
4 * 62000, SDS-PAGE
Hyphomicrobium methylovorum
4.1.3.1
250000
-
gel filtration
Hyphomicrobium methylovorum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Hyphomicrobium methylovorum
assimilation of one-carbon compounds in the icl+ serine pathway
?
-
-
-
4.1.3.1
isocitrate
Hyphomicrobium methylovorum
glyoxylate cycle
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Hyphomicrobium methylovorum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
2.4
-
-
Hyphomicrobium methylovorum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33148
Hyphomicrobium methylovorum
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33148
Hyphomicrobium methylovorum
succinate + glyoxylate
-
33148
Hyphomicrobium methylovorum
-
4.1.3.1
isocitrate
assimilation of one-carbon compounds in the icl+ serine pathway
33148
Hyphomicrobium methylovorum
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33148
Hyphomicrobium methylovorum
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 62000, SDS-PAGE
Hyphomicrobium methylovorum
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.3.1
45
-
-
Hyphomicrobium methylovorum
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
25
-
no loss of activity after 30 min
Hyphomicrobium methylovorum
4.1.3.1
30
-
16% loss of activity after 30 min
Hyphomicrobium methylovorum
4.1.3.1
40
-
30% loss of activity after 30 min
Hyphomicrobium methylovorum
4.1.3.1
50
-
82% loss of activity after 30 min
Hyphomicrobium methylovorum
4.1.3.1
60
-
100% loss of activity after 30 min
Hyphomicrobium methylovorum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.5
-
-
Hyphomicrobium methylovorum
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.1.3.1
7.5
9
at 15°C stable for 30 min
Hyphomicrobium methylovorum