BRENDA - Enzyme Database

The purification and physicochemical characterization of maize (Zea mays L.) isocitrate lyase

Khan, A.S.; VanDriessche, E.; Kanarek, L.; Beeckmans, S.; Arch. Biochem. Biophys. 297, 9-18 (1992)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
Ba2+
no inhibition
Zea mays
4.1.3.1
Ca2+
no inhibition
Zea mays
4.1.3.1
Sr2+
no inhibition
Zea mays
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.06
-
isocitrate
100 mM Hepes, pH 7.0
Zea mays
4.1.3.1
0.52
-
isocitrate
60 mM potassium phosphate, pH 7.5
Zea mays
4.1.3.1
0.76
-
isocitrate
60 mM triethanolamine, pH 7.5
Zea mays
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Ba2+
no activation
Zea mays
4.1.3.1
Mg2+
activity of demetallized enzyme: 0.5%
Zea mays
4.1.3.1
Mn2+
Mn2+ 30% as effective as Mg2+
Zea mays
4.1.3.1
Sr2+
no activation
Zea mays
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
64000
-
4 * 64000, SDS-PAGE
Zea mays
4.1.3.1
252000
-
gel filtration
Zea mays
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Zea mays
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Zea mays
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
4.1.3.1
no modification
-
Zea mays
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Zea mays
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
scutellum
-
Zea mays
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
4.96
-
-
Zea mays
Storage Stability
EC Number
Storage Stability
Organism
4.1.3.1
-70°C, concentrated to 2.4 mg/ml, 10 mM triethanolamine buffer, 12 mM MgCl2, 14 mM 2-mercaptoethanol, pH 7.6, 50% v/v glycerol, several months, stable
Zea mays
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33147
Zea mays
succinate + glyoxylate
-
33147
Zea mays
r
4.1.3.1
isocitrate
-
33147
Zea mays
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 64000, SDS-PAGE
Zea mays
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.2
-
-
Zea mays
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
Ba2+
no inhibition
Zea mays
4.1.3.1
Ca2+
no inhibition
Zea mays
4.1.3.1
Sr2+
no inhibition
Zea mays
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.06
-
isocitrate
100 mM Hepes, pH 7.0
Zea mays
4.1.3.1
0.52
-
isocitrate
60 mM potassium phosphate, pH 7.5
Zea mays
4.1.3.1
0.76
-
isocitrate
60 mM triethanolamine, pH 7.5
Zea mays
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Ba2+
no activation
Zea mays
4.1.3.1
Mg2+
activity of demetallized enzyme: 0.5%
Zea mays
4.1.3.1
Mn2+
Mn2+ 30% as effective as Mg2+
Zea mays
4.1.3.1
Sr2+
no activation
Zea mays
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
64000
-
4 * 64000, SDS-PAGE
Zea mays
4.1.3.1
252000
-
gel filtration
Zea mays
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Zea mays
-
?
-
-
-
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
4.1.3.1
no modification
-
Zea mays
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Zea mays
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
scutellum
-
Zea mays
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
4.96
-
-
Zea mays
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.3.1
-70°C, concentrated to 2.4 mg/ml, 10 mM triethanolamine buffer, 12 mM MgCl2, 14 mM 2-mercaptoethanol, pH 7.6, 50% v/v glycerol, several months, stable
Zea mays
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33147
Zea mays
succinate + glyoxylate
-
33147
Zea mays
r
4.1.3.1
isocitrate
-
33147
Zea mays
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 64000, SDS-PAGE
Zea mays
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.2
-
-
Zea mays