BRENDA - Enzyme Database

Purification and characterization of Acinetobacter calcoaceticus isocitrate lyase

Hoyt, J.C.; Johnson, K.E.; Reeves, H.C.; J. Bacteriol. 173, 6844-6848 (1991)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
Ba2+
-
Acinetobacter calcoaceticus
4.1.3.1
Ca2+
-
Acinetobacter calcoaceticus
4.1.3.1
glycolate
-
Acinetobacter calcoaceticus
4.1.3.1
Hydroxymalonate
-
Acinetobacter calcoaceticus
4.1.3.1
malonate
-
Acinetobacter calcoaceticus
4.1.3.1
methylenesuccinate
-
Acinetobacter calcoaceticus
4.1.3.1
oxalate
-
Acinetobacter calcoaceticus
4.1.3.1
Sr2+
-
Acinetobacter calcoaceticus
4.1.3.1
Tartrate
-
Acinetobacter calcoaceticus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.04
-
threo-Ds-isocitrate
-
Acinetobacter calcoaceticus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Co2+
Co2+ 12% as effective as Mg2+; no activity in absence of exogenous cation
Acinetobacter calcoaceticus
4.1.3.1
Fe2+
no activity in absence of exogenous cation, Fe2+ 7% as effective as Mg2+
Acinetobacter calcoaceticus
4.1.3.1
Mg2+
increase of activity
Acinetobacter calcoaceticus
4.1.3.1
Mn2+
Mn2+ 14% as effective as Mg2+; no activity in absence of exogenous cation
Acinetobacter calcoaceticus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
64000
-
4 * 64000, SDS-PAGE
Acinetobacter calcoaceticus
4.1.3.1
250000
-
gel filtration
Acinetobacter calcoaceticus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Acinetobacter calcoaceticus
glyoxylate cycle
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Acinetobacter calcoaceticus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Acinetobacter calcoaceticus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
2.9
-
-
Acinetobacter calcoaceticus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33140
Acinetobacter calcoaceticus
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33140
Acinetobacter calcoaceticus
succinate + glyoxylate
-
33140
Acinetobacter calcoaceticus
-
4.1.3.1
isocitrate
glyoxylate cycle
33140
Acinetobacter calcoaceticus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 64000, SDS-PAGE
Acinetobacter calcoaceticus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.5
-
-
Acinetobacter calcoaceticus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
Ba2+
-
Acinetobacter calcoaceticus
4.1.3.1
Ca2+
-
Acinetobacter calcoaceticus
4.1.3.1
glycolate
-
Acinetobacter calcoaceticus
4.1.3.1
Hydroxymalonate
-
Acinetobacter calcoaceticus
4.1.3.1
malonate
-
Acinetobacter calcoaceticus
4.1.3.1
methylenesuccinate
-
Acinetobacter calcoaceticus
4.1.3.1
oxalate
-
Acinetobacter calcoaceticus
4.1.3.1
Sr2+
-
Acinetobacter calcoaceticus
4.1.3.1
Tartrate
-
Acinetobacter calcoaceticus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.04
-
threo-Ds-isocitrate
-
Acinetobacter calcoaceticus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Co2+
Co2+ 12% as effective as Mg2+; no activity in absence of exogenous cation
Acinetobacter calcoaceticus
4.1.3.1
Fe2+
no activity in absence of exogenous cation, Fe2+ 7% as effective as Mg2+
Acinetobacter calcoaceticus
4.1.3.1
Mg2+
increase of activity
Acinetobacter calcoaceticus
4.1.3.1
Mn2+
Mn2+ 14% as effective as Mg2+; no activity in absence of exogenous cation
Acinetobacter calcoaceticus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
64000
-
4 * 64000, SDS-PAGE
Acinetobacter calcoaceticus
4.1.3.1
250000
-
gel filtration
Acinetobacter calcoaceticus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Acinetobacter calcoaceticus
glyoxylate cycle
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Acinetobacter calcoaceticus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
2.9
-
-
Acinetobacter calcoaceticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33140
Acinetobacter calcoaceticus
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33140
Acinetobacter calcoaceticus
succinate + glyoxylate
-
33140
Acinetobacter calcoaceticus
-
4.1.3.1
isocitrate
glyoxylate cycle
33140
Acinetobacter calcoaceticus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 64000, SDS-PAGE
Acinetobacter calcoaceticus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.5
-
-
Acinetobacter calcoaceticus