BRENDA - Enzyme Database

Purification and properties of isocitrate lyase from flax seedlings

Khan, F.R.; Sallemuddin, M.; Siddiqi, M; McFadden, B.A.; Arch. Biochem. Biophys. 183, 13-23 (1977)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
cysteine
increase of activity
Linum usitatissimum
4.1.3.1
sulfhydryl compounds
increase of activity
Linum usitatissimum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
glyoxylate
-
Linum usitatissimum
4.1.3.1
malonate
-
Linum usitatissimum
4.1.3.1
sulfhydryl compounds
increase of activity
Linum usitatissimum
4.1.3.1
Tartrate
-
Linum usitatissimum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Co2+
Co2+ 29% as effective as Mg2+; no activity in absence of exogenous cation
Linum usitatissimum
4.1.3.1
Mg2+
increase of activity
Linum usitatissimum
4.1.3.1
Mn2+
Mn2+ 24% as effective as Mg2+; no activity in absence of exogenous cation
Linum usitatissimum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
264000
-
gel filtration
Linum usitatissimum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Linum usitatissimum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Linum usitatissimum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
seedling
-
Linum usitatissimum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33139
Linum usitatissimum
succinate + glyoxylate
-
33139
Linum usitatissimum
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4*67000, SDS-PAGE
Linum usitatissimum
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.3.1
30
-
-
Linum usitatissimum
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.1.3.1
15
40
-
Linum usitatissimum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.5
-
phosphate, Tris or imidazole buffer
Linum usitatissimum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.3.1
5
10
-
Linum usitatissimum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
cysteine
increase of activity
Linum usitatissimum
4.1.3.1
sulfhydryl compounds
increase of activity
Linum usitatissimum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
glyoxylate
-
Linum usitatissimum
4.1.3.1
malonate
-
Linum usitatissimum
4.1.3.1
sulfhydryl compounds
increase of activity
Linum usitatissimum
4.1.3.1
Tartrate
-
Linum usitatissimum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Co2+
Co2+ 29% as effective as Mg2+; no activity in absence of exogenous cation
Linum usitatissimum
4.1.3.1
Mg2+
increase of activity
Linum usitatissimum
4.1.3.1
Mn2+
Mn2+ 24% as effective as Mg2+; no activity in absence of exogenous cation
Linum usitatissimum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
264000
-
gel filtration
Linum usitatissimum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Linum usitatissimum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
seedling
-
Linum usitatissimum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33139
Linum usitatissimum
succinate + glyoxylate
-
33139
Linum usitatissimum
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4*67000, SDS-PAGE
Linum usitatissimum
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.3.1
30
-
-
Linum usitatissimum
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.1.3.1
15
40
-
Linum usitatissimum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.5
-
phosphate, Tris or imidazole buffer
Linum usitatissimum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.3.1
5
10
-
Linum usitatissimum