BRENDA - Enzyme Database

Isocitrate lyase from Neurospora crassa. I. Purification, kinetic mechanism, and interaction with inhibitors

Johanson, R.A.; Hill, J.M.; McFadden, B.A.; Biochim. Biophys. Acta 364, 327-340 (1974)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
thiol compounds
increase of activity
Neurospora crassa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
Cl-
-
Neurospora crassa
4.1.3.1
Cl-
-
Vogesella indigofera
4.1.3.1
fructose-1,6-bisphosphate
-
Neurospora crassa
4.1.3.1
fumarate
-
Neurospora crassa
4.1.3.1
fumarate
-
Vogesella indigofera
4.1.3.1
glyoxylate
-
Neurospora crassa
4.1.3.1
HPO42-
-
Neurospora crassa
4.1.3.1
HPO42-
-
Vogesella indigofera
4.1.3.1
malate
-
Neurospora crassa
4.1.3.1
malate
-
Vogesella indigofera
4.1.3.1
NO3-
-
Neurospora crassa
4.1.3.1
NO3-
-
Vogesella indigofera
4.1.3.1
phosphoenolpyruvate
-
Neurospora crassa
4.1.3.1
SO42-
-
Neurospora crassa
4.1.3.1
SO42-
-
Vogesella indigofera
4.1.3.1
succinate
-
Neurospora crassa
4.1.3.1
succinate
-
Vogesella indigofera
4.1.3.1
threo-DL-homoisocitrate
-
Neurospora crassa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.014
-
glyoxylate
-
Neurospora crassa
4.1.3.1
0.044
-
succinate
-
Neurospora crassa
4.1.3.1
0.05
-
threo-Ds-isocitrate
-
Neurospora crassa
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
increase of activity
Neurospora crassa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Neurospora crassa
glyoxylate cycle
?
-
-
-
4.1.3.1
isocitrate
Vogesella indigofera
glyoxylate cycle
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Neurospora crassa
-
-
-
4.1.3.1
Vogesella indigofera
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Neurospora crassa
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
16
-
-
Neurospora crassa
Storage Stability
EC Number
Storage Stability
Organism
4.1.3.1
-80°C, 6 months, stable
Neurospora crassa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33137
Neurospora crassa
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33137
Neurospora crassa
succinate + glyoxylate
-
33137
Neurospora crassa
r
4.1.3.1
isocitrate
-
33137
Vogesella indigofera
succinate + glyoxylate
-
33137
Vogesella indigofera
r
4.1.3.1
isocitrate
glyoxylate cycle
33137
Neurospora crassa
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33137
Vogesella indigofera
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.8
-
-
Neurospora crassa
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
thiol compounds
increase of activity
Neurospora crassa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
Cl-
-
Neurospora crassa
4.1.3.1
Cl-
-
Vogesella indigofera
4.1.3.1
fructose-1,6-bisphosphate
-
Neurospora crassa
4.1.3.1
fumarate
-
Neurospora crassa
4.1.3.1
fumarate
-
Vogesella indigofera
4.1.3.1
glyoxylate
-
Neurospora crassa
4.1.3.1
HPO42-
-
Neurospora crassa
4.1.3.1
HPO42-
-
Vogesella indigofera
4.1.3.1
malate
-
Neurospora crassa
4.1.3.1
malate
-
Vogesella indigofera
4.1.3.1
NO3-
-
Neurospora crassa
4.1.3.1
NO3-
-
Vogesella indigofera
4.1.3.1
phosphoenolpyruvate
-
Neurospora crassa
4.1.3.1
SO42-
-
Neurospora crassa
4.1.3.1
SO42-
-
Vogesella indigofera
4.1.3.1
succinate
-
Neurospora crassa
4.1.3.1
succinate
-
Vogesella indigofera
4.1.3.1
threo-DL-homoisocitrate
-
Neurospora crassa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.014
-
glyoxylate
-
Neurospora crassa
4.1.3.1
0.044
-
succinate
-
Neurospora crassa
4.1.3.1
0.05
-
threo-Ds-isocitrate
-
Neurospora crassa
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
increase of activity
Neurospora crassa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Neurospora crassa
glyoxylate cycle
?
-
-
-
4.1.3.1
isocitrate
Vogesella indigofera
glyoxylate cycle
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Neurospora crassa
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
16
-
-
Neurospora crassa
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.3.1
-80°C, 6 months, stable
Neurospora crassa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33137
Neurospora crassa
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33137
Neurospora crassa
succinate + glyoxylate
-
33137
Neurospora crassa
r
4.1.3.1
isocitrate
-
33137
Vogesella indigofera
succinate + glyoxylate
-
33137
Vogesella indigofera
r
4.1.3.1
isocitrate
glyoxylate cycle
33137
Neurospora crassa
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33137
Vogesella indigofera
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.8
-
-
Neurospora crassa