BRENDA - Enzyme Database

Isocitrate lyase from the free-living nematode, Tubatrix aceti: purification and properties

Reiss, U.; Rothstein, M.; Biochemistry 13, 1796-1800 (1974)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
cysteine
increase of activity
Turbatrix aceti
4.1.3.1
sulfhydryl compounds
increase of activity
Turbatrix aceti
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
malonate
-
Turbatrix aceti
4.1.3.1
p-hydroxymercuribenzoate
complete inhibition at 0.016 mM
Turbatrix aceti
4.1.3.1
sulfhydryl compounds
increase of activity
Turbatrix aceti
4.1.3.1
Tartrate
-
Turbatrix aceti
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.066
-
Ds-isocitrate
-
Turbatrix aceti
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
increase of activity
Turbatrix aceti
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
123000
-
4 * 123000, SDS-PAGE
Turbatrix aceti
4.1.3.1
480000
-
gel filtration
Turbatrix aceti
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Turbatrix aceti
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Turbatrix aceti
Storage Stability
EC Number
Storage Stability
Organism
4.1.3.1
-20°C, 4 weeks, 25-30% loss of activity
Turbatrix aceti
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33136
Turbatrix aceti
succinate + glyoxylate
-
33136
Turbatrix aceti
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 123000, SDS-PAGE
Turbatrix aceti
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.3.1
800
-
isocitrate
-
Turbatrix aceti
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.6
-
-
Turbatrix aceti
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
cysteine
increase of activity
Turbatrix aceti
4.1.3.1
sulfhydryl compounds
increase of activity
Turbatrix aceti
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
malonate
-
Turbatrix aceti
4.1.3.1
p-hydroxymercuribenzoate
complete inhibition at 0.016 mM
Turbatrix aceti
4.1.3.1
sulfhydryl compounds
increase of activity
Turbatrix aceti
4.1.3.1
Tartrate
-
Turbatrix aceti
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.066
-
Ds-isocitrate
-
Turbatrix aceti
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
increase of activity
Turbatrix aceti
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
123000
-
4 * 123000, SDS-PAGE
Turbatrix aceti
4.1.3.1
480000
-
gel filtration
Turbatrix aceti
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Turbatrix aceti
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.3.1
-20°C, 4 weeks, 25-30% loss of activity
Turbatrix aceti
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33136
Turbatrix aceti
succinate + glyoxylate
-
33136
Turbatrix aceti
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 123000, SDS-PAGE
Turbatrix aceti
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.3.1
800
-
isocitrate
-
Turbatrix aceti
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.6
-
-
Turbatrix aceti