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Literature summary extracted from
- Isocitrate lyase from the free-living nematode, Tubatrix aceti: purification and properties (1974), Biochemistry, 13, 1796-1800.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.1 | cysteine | increase of activity | Turbatrix aceti |  |
| 4.1.3.1 | sulfhydryl compounds | increase of activity | Turbatrix aceti | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.1 | malonate | - |
Turbatrix aceti | |
| 4.1.3.1 | p-hydroxymercuribenzoate | complete inhibition at 0.016 mM | Turbatrix aceti | |
| 4.1.3.1 | sulfhydryl compounds | increase of activity | Turbatrix aceti | |
| 4.1.3.1 | Tartrate | - |
Turbatrix aceti | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.3.1 | 0.066 | - |
Ds-isocitrate | - |
Turbatrix aceti | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.1 | Mg2+ | increase of activity | Turbatrix aceti | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 123000 | - |
4 * 123000, SDS-PAGE | Turbatrix aceti |
| 4.1.3.1 | 480000 | - |
gel filtration | Turbatrix aceti |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.3.1 | Turbatrix aceti | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.3.1 | - |
Turbatrix aceti |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.1.3.1 | -20°C, 4 weeks, 25-30% loss of activity | Turbatrix aceti |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.3.1 | isocitrate | - |
Turbatrix aceti | succinate + glyoxylate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.3.1 | tetramer | 4 * 123000, SDS-PAGE | Turbatrix aceti |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.3.1 | 800 | - |
isocitrate | - |
Turbatrix aceti | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 7.6 | - |
- |
Turbatrix aceti |
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