BRENDA - Enzyme Database

Isocitrate lyase from a thermophilic Bacillus: effect of salts on enzyme activity

Griffiths, M.W.; Sundaram, T.K.; J. Bacteriol. 116, 1160-1169 (1973)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
KCl
at pH-values above 8.6 inhibition, below pH 8.6 activation by KCl
Bacillus sp. (in: Bacteria)
4.1.3.1
NaCl
activation by NaCl
Bacillus sp. (in: Bacteria)
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
-
Bacillus sp. (in: Bacteria)
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Bacillus sp. (in: Bacteria)
-
thermophilic
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33134
Bacillus sp. (in: Bacteria)
succinate + glyoxylate
-
33134
Bacillus sp. (in: Bacteria)
r
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
additional information
-
KCl, MgCl2, CaCl2 stabilize
Bacillus sp. (in: Bacteria)
4.1.3.1
55.5
-
50% activity lost after 43 min, in presence of 0.4 M KCl 50% activity lost after 129 min
Bacillus sp. (in: Bacteria)
4.1.3.1
60
-
50% activity lost after 5 min, in presence of 0.4 M KCl 50% activity lost after 4 min
Bacillus sp. (in: Bacteria)
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.5
-
presence of 0.3 M KCl
Bacillus sp. (in: Bacteria)
4.1.3.1
8
-
absence of KCl
Bacillus sp. (in: Bacteria)
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
KCl
at pH-values above 8.6 inhibition, below pH 8.6 activation by KCl
Bacillus sp. (in: Bacteria)
4.1.3.1
NaCl
activation by NaCl
Bacillus sp. (in: Bacteria)
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
-
Bacillus sp. (in: Bacteria)
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33134
Bacillus sp. (in: Bacteria)
succinate + glyoxylate
-
33134
Bacillus sp. (in: Bacteria)
r
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
additional information
-
KCl, MgCl2, CaCl2 stabilize
Bacillus sp. (in: Bacteria)
4.1.3.1
55.5
-
50% activity lost after 43 min, in presence of 0.4 M KCl 50% activity lost after 129 min
Bacillus sp. (in: Bacteria)
4.1.3.1
60
-
50% activity lost after 5 min, in presence of 0.4 M KCl 50% activity lost after 4 min
Bacillus sp. (in: Bacteria)
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.5
-
presence of 0.3 M KCl
Bacillus sp. (in: Bacteria)
4.1.3.1
8
-
absence of KCl
Bacillus sp. (in: Bacteria)