BRENDA - Enzyme Database

Plant microbody proteins. Purification and glycoprotein nature of glyoxysomal isocitrate lyase from cucumber cotyledons

Frevert, J.; Kindl, H.; Eur. J. Biochem. 92, 35-43 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
SO42-
-
Cucumis sativus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.1
-
threo-Ds-isocitrate
Hepes, pH 7.4
Cucumis sativus
4.1.3.1
0.26
-
threo-Ds-isocitrate
Hepes, pH 7.4, 60 mM Cl-
Cucumis sativus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.3.1
glyoxysome
matrix
Cucumis sativus
9514
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
64000
-
4 * 64000, SDS-PAGE
Cucumis sativus
4.1.3.1
255000
-
gel filtration
Cucumis sativus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Cucumis sativus
glyoxylate cycle
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Cucumis sativus
-
cucumber
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
4.1.3.1
side-chain modification
2% neutral sugar
Cucumis sativus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
cotyledon
-
Cucumis sativus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33132
Cucumis sativus
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33132
Cucumis sativus
succinate + glyoxylate
-
33132
Cucumis sativus
r
4.1.3.1
isocitrate
glyoxylate cycle
33132
Cucumis sativus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 64000, SDS-PAGE
Cucumis sativus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.2
7.6
-
Cucumis sativus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.3.1
6.9
7.9
50% of optimal activity at pH 6.9 and 7.9
Cucumis sativus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
SO42-
-
Cucumis sativus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.1
-
threo-Ds-isocitrate
Hepes, pH 7.4
Cucumis sativus
4.1.3.1
0.26
-
threo-Ds-isocitrate
Hepes, pH 7.4, 60 mM Cl-
Cucumis sativus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.3.1
glyoxysome
matrix
Cucumis sativus
9514
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
64000
-
4 * 64000, SDS-PAGE
Cucumis sativus
4.1.3.1
255000
-
gel filtration
Cucumis sativus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Cucumis sativus
glyoxylate cycle
?
-
-
-
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
4.1.3.1
side-chain modification
2% neutral sugar
Cucumis sativus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
cotyledon
-
Cucumis sativus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33132
Cucumis sativus
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33132
Cucumis sativus
succinate + glyoxylate
-
33132
Cucumis sativus
r
4.1.3.1
isocitrate
glyoxylate cycle
33132
Cucumis sativus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 64000, SDS-PAGE
Cucumis sativus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.2
7.6
-
Cucumis sativus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.3.1
6.9
7.9
50% of optimal activity at pH 6.9 and 7.9
Cucumis sativus