BRENDA - Enzyme Database

Isolation and properties of isocitrate lyase from Lupinus seed

Vicenzini, M.T.; Nerozzi, F.; Vincieri, F.F.; Vanni, P.; Phytochemistry 19, 769-774 (1980)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
dithiothreitol
increase of activity
Lupinus sp.
4.1.3.1
Na2S2O4
increase of activity
Lupinus sp.
4.1.3.1
Reducing agents
increase of activity
Lupinus sp.
4.1.3.1
thioglycolate
increase of activity
Lupinus sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
glyoxylate
-
Lupinus sp.
4.1.3.1
malonate
-
Lupinus sp.
4.1.3.1
N-ethylmaleimide
-
Lupinus sp.
4.1.3.1
oxalate
-
Lupinus sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.05
-
glyoxylate
-
Lupinus sp.
4.1.3.1
0.1
0.2
isocitrate
Tris, triethanolamine or imidazole buffer
Lupinus sp.
4.1.3.1
0.2
-
succinate
-
Lupinus sp.
4.1.3.1
1
-
isocitrate
phosphate buffer, pH 6.8 or 7.5
Lupinus sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
increase of activity
Lupinus sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
145000
170000
gel filtration
Lupinus sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Lupinus sp.
glyoxylate cycle
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Lupinus sp.
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Lupinus sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
seed
-
Lupinus sp.
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.1.3.1
1.83
-
-
Lupinus sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33130
Lupinus sp.
succinate + glyoxylate
-
33130
Lupinus sp.
r
4.1.3.1
isocitrate
glyoxylate cycle
33130
Lupinus sp.
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7
-
triethanolamine buffer
Lupinus sp.
4.1.3.1
7.5
-
phosphate, Tris or imidazole buffer
Lupinus sp.
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
dithiothreitol
increase of activity
Lupinus sp.
4.1.3.1
Na2S2O4
increase of activity
Lupinus sp.
4.1.3.1
Reducing agents
increase of activity
Lupinus sp.
4.1.3.1
thioglycolate
increase of activity
Lupinus sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
glyoxylate
-
Lupinus sp.
4.1.3.1
malonate
-
Lupinus sp.
4.1.3.1
N-ethylmaleimide
-
Lupinus sp.
4.1.3.1
oxalate
-
Lupinus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.05
-
glyoxylate
-
Lupinus sp.
4.1.3.1
0.1
0.2
isocitrate
Tris, triethanolamine or imidazole buffer
Lupinus sp.
4.1.3.1
0.2
-
succinate
-
Lupinus sp.
4.1.3.1
1
-
isocitrate
phosphate buffer, pH 6.8 or 7.5
Lupinus sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
increase of activity
Lupinus sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
145000
170000
gel filtration
Lupinus sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Lupinus sp.
glyoxylate cycle
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Lupinus sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
seed
-
Lupinus sp.
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.1.3.1
1.83
-
-
Lupinus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33130
Lupinus sp.
succinate + glyoxylate
-
33130
Lupinus sp.
r
4.1.3.1
isocitrate
glyoxylate cycle
33130
Lupinus sp.
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7
-
triethanolamine buffer
Lupinus sp.
4.1.3.1
7.5
-
phosphate, Tris or imidazole buffer
Lupinus sp.