BRENDA - Enzyme Database

Isolation and characterization of isocitrate lyase of castor endosperm

Malhotra, O.P.; Srivastava, P.K.; Arch. Biochem. Biophys. 214, 164-171 (1982)

Data extracted from this reference:

Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Ba2+
partial reactivation after thermal inactivation
Ricinus communis
4.1.3.1
Mg2+
increase of activity
Ricinus communis
4.1.3.1
Mn2+
partial reactivation after thermal inactivation
Ricinus communis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
35000
-
4 * 35000, SDS-PAGE
Ricinus communis
4.1.3.1
140000
-
gel filtration
Ricinus communis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Ricinus communis
glyoxylate cycle
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Ricinus communis
-
castor bean
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Ricinus communis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
endosperm
-
Ricinus communis
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
2.7
-
-
Ricinus communis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33129
Ricinus communis
succinate + glyoxylate
-
33129
Ricinus communis
r
4.1.3.1
isocitrate
glyoxylate cycle
33129
Ricinus communis
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 35000, SDS-PAGE
Ricinus communis
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
additional information
-
biphasic kinetics of thermal inactivation
Ricinus communis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.2
7.3
-
Ricinus communis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Ba2+
partial reactivation after thermal inactivation
Ricinus communis
4.1.3.1
Mg2+
increase of activity
Ricinus communis
4.1.3.1
Mn2+
partial reactivation after thermal inactivation
Ricinus communis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
35000
-
4 * 35000, SDS-PAGE
Ricinus communis
4.1.3.1
140000
-
gel filtration
Ricinus communis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Ricinus communis
glyoxylate cycle
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Ricinus communis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
endosperm
-
Ricinus communis
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
2.7
-
-
Ricinus communis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33129
Ricinus communis
succinate + glyoxylate
-
33129
Ricinus communis
r
4.1.3.1
isocitrate
glyoxylate cycle
33129
Ricinus communis
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 35000, SDS-PAGE
Ricinus communis
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
additional information
-
biphasic kinetics of thermal inactivation
Ricinus communis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.2
7.3
-
Ricinus communis