BRENDA - Enzyme Database

Purification and some properties of isocitrate lyase from Paecilomyces varioti with high malic acid production

Takao, S.; Takahashi, T.; Tanida, M.; Takahashi, M.; J. Ferment. Technol. 62, 577-582 (1984)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
2-oxoglutarate
-
Byssochlamys spectabilis
4.1.3.1
citrate
no inhibition at 0.1 mM
Byssochlamys spectabilis
4.1.3.1
fumarate
no inhibition at 0.1 mM
Byssochlamys spectabilis
4.1.3.1
malate
no inhibition at 0.1 mM
Byssochlamys spectabilis
4.1.3.1
oxalacetate
-
Byssochlamys spectabilis
4.1.3.1
phosphoenolpyruvate
-
Byssochlamys spectabilis
4.1.3.1
pyruvate
-
Byssochlamys spectabilis
4.1.3.1
succinate
-
Byssochlamys spectabilis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.018
-
threo-Ds-isocitrate
-
Byssochlamys spectabilis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
increase of activity
Byssochlamys spectabilis
4.1.3.1
Mn2+
Mn2+ less effective activator than Mg2+
Byssochlamys spectabilis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
240000
-
gel filtration
Byssochlamys spectabilis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Byssochlamys spectabilis
glyoxylate cycle
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Byssochlamys spectabilis
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
4.1.3.1
dithiothreitol very effective in protecting enzyme from loss of activity
Byssochlamys spectabilis
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Byssochlamys spectabilis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
103
-
-
Byssochlamys spectabilis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33124
Byssochlamys spectabilis
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33124
Byssochlamys spectabilis
succinate + glyoxylate
-
33124
Byssochlamys spectabilis
r
4.1.3.1
isocitrate
glyoxylate cycle
33124
Byssochlamys spectabilis
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4*60000, SDS-PAGE
Byssochlamys spectabilis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.3.1
37
-
-
Byssochlamys spectabilis
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.1.3.1
20
50
-
Byssochlamys spectabilis
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
65
-
40% activity lost after 15 min
Byssochlamys spectabilis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.2
-
-
Byssochlamys spectabilis
4.1.3.1
7.5
-
-
Byssochlamys spectabilis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.3.1
6.2
8
-
Byssochlamys spectabilis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
2-oxoglutarate
-
Byssochlamys spectabilis
4.1.3.1
citrate
no inhibition at 0.1 mM
Byssochlamys spectabilis
4.1.3.1
fumarate
no inhibition at 0.1 mM
Byssochlamys spectabilis
4.1.3.1
malate
no inhibition at 0.1 mM
Byssochlamys spectabilis
4.1.3.1
oxalacetate
-
Byssochlamys spectabilis
4.1.3.1
phosphoenolpyruvate
-
Byssochlamys spectabilis
4.1.3.1
pyruvate
-
Byssochlamys spectabilis
4.1.3.1
succinate
-
Byssochlamys spectabilis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.018
-
threo-Ds-isocitrate
-
Byssochlamys spectabilis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
increase of activity
Byssochlamys spectabilis
4.1.3.1
Mn2+
Mn2+ less effective activator than Mg2+
Byssochlamys spectabilis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
240000
-
gel filtration
Byssochlamys spectabilis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Byssochlamys spectabilis
glyoxylate cycle
?
-
-
-
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
4.1.3.1
dithiothreitol very effective in protecting enzyme from loss of activity
Byssochlamys spectabilis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Byssochlamys spectabilis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
103
-
-
Byssochlamys spectabilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33124
Byssochlamys spectabilis
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33124
Byssochlamys spectabilis
succinate + glyoxylate
-
33124
Byssochlamys spectabilis
r
4.1.3.1
isocitrate
glyoxylate cycle
33124
Byssochlamys spectabilis
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4*60000, SDS-PAGE
Byssochlamys spectabilis
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.3.1
37
-
-
Byssochlamys spectabilis
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.1.3.1
20
50
-
Byssochlamys spectabilis
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
65
-
40% activity lost after 15 min
Byssochlamys spectabilis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.2
-
-
Byssochlamys spectabilis
4.1.3.1
7.5
-
-
Byssochlamys spectabilis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.3.1
6.2
8
-
Byssochlamys spectabilis