BRENDA - Enzyme Database

Partial purification of isocitrate lyase from Candida tropicalis and some kinetic properties of the enzyme

Tanaka, A.; Nabeshima, S.; Tokuda, M.; Fukui, S.; Agric. Biol. Chem. 41, 795-801 (1977)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
2-oxoglutarate
19% inhibition at 5 mM
Candida tropicalis
4.1.3.1
citrate
22% inhibition at 5 mM
Candida tropicalis
4.1.3.1
fructose-1,6-bisphosphate
39% inhibition at 5 mM
Candida tropicalis
4.1.3.1
glucose-6-phosphate
44% inhibition at 5 mM
Candida tropicalis
4.1.3.1
oxalacetate
50% inhibition at 5 mM
Candida tropicalis
4.1.3.1
phosphoenolpyruvate
-
Candida tropicalis
4.1.3.1
pyruvate
58% inhibition at 5 mM
Candida tropicalis
4.1.3.1
succinate
21% inhibition at 5 mM
Candida tropicalis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
1.2
1.7
DL-isocitrate
-
Candida tropicalis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Candida tropicalis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
partial
Candida tropicalis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
9.9
11
-
Candida tropicalis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33120
Candida tropicalis
succinate + glyoxylate
-
33120
Candida tropicalis
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.8
7
-
Candida tropicalis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.3.1
5.5
7.8
-
Candida tropicalis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.1.3.1
5
-
oxalacetate
-
Candida tropicalis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
2-oxoglutarate
19% inhibition at 5 mM
Candida tropicalis
4.1.3.1
citrate
22% inhibition at 5 mM
Candida tropicalis
4.1.3.1
fructose-1,6-bisphosphate
39% inhibition at 5 mM
Candida tropicalis
4.1.3.1
glucose-6-phosphate
44% inhibition at 5 mM
Candida tropicalis
4.1.3.1
oxalacetate
50% inhibition at 5 mM
Candida tropicalis
4.1.3.1
phosphoenolpyruvate
-
Candida tropicalis
4.1.3.1
pyruvate
58% inhibition at 5 mM
Candida tropicalis
4.1.3.1
succinate
21% inhibition at 5 mM
Candida tropicalis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.1.3.1
5
-
oxalacetate
-
Candida tropicalis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
1.2
1.7
DL-isocitrate
-
Candida tropicalis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
partial
Candida tropicalis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
9.9
11
-
Candida tropicalis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33120
Candida tropicalis
succinate + glyoxylate
-
33120
Candida tropicalis
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.8
7
-
Candida tropicalis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.3.1
5.5
7.8
-
Candida tropicalis