BRENDA - Enzyme Database

Purification and regulatory properties of isocitrate lyase from Escherichia coli ML308

Mackintosh, C.; Nimmo, H.G.; Biochem. J. 250, 25-31 (1988)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
2-oxoglutarate
-
Escherichia coli
4.1.3.1
3-phosphoglycerate
-
Escherichia coli
4.1.3.1
Cl-
-
Escherichia coli
4.1.3.1
glycolate
-
Escherichia coli
4.1.3.1
phosphate
-
Escherichia coli
4.1.3.1
phosphoenolpyruvate
-
Escherichia coli
4.1.3.1
SO42-
-
Escherichia coli
4.1.3.1
succinate
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.062
-
Ds-isocitrate
pH 7.3
Escherichia coli
4.1.3.1
0.13
-
glyoxylate
-
Escherichia coli
4.1.3.1
0.59
-
succinate
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
44700
-
4 * 44700, SDS-PAGE
Escherichia coli
4.1.3.1
177000
-
gel filtration
Escherichia coli
4.1.3.1
180000
-
sedimentation equilibrium centrifugation
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.1.3.1
37.5
-
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
4.1.3.1
-20░C, at least 6 months, stable
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33118
Escherichia coli
succinate + glyoxylate
-
33118
Escherichia coli
r
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 44700, SDS-PAGE
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
2-oxoglutarate
-
Escherichia coli
4.1.3.1
3-phosphoglycerate
-
Escherichia coli
4.1.3.1
Cl-
-
Escherichia coli
4.1.3.1
glycolate
-
Escherichia coli
4.1.3.1
phosphate
-
Escherichia coli
4.1.3.1
phosphoenolpyruvate
-
Escherichia coli
4.1.3.1
SO42-
-
Escherichia coli
4.1.3.1
succinate
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.062
-
Ds-isocitrate
pH 7.3
Escherichia coli
4.1.3.1
0.13
-
glyoxylate
-
Escherichia coli
4.1.3.1
0.59
-
succinate
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
44700
-
4 * 44700, SDS-PAGE
Escherichia coli
4.1.3.1
177000
-
gel filtration
Escherichia coli
4.1.3.1
180000
-
sedimentation equilibrium centrifugation
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.1.3.1
37.5
-
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.3.1
-20░C, at least 6 months, stable
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
33118
Escherichia coli
succinate + glyoxylate
-
33118
Escherichia coli
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 44700, SDS-PAGE
Escherichia coli