BRENDA - Enzyme Database

Purification and characterization of isocitrate lyase from Escherichia coli

Robertson, E.F.; Reeves, H.C.; Curr. Microbiol. 14, 347-350 (1987)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.008
-
threo-Ds-isocitrate
-
Escherichia coli
4.1.3.1
0.015
-
DL-isocitrate
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
48000
-
4 * 48000, SDS-PAGE
Escherichia coli
4.1.3.1
188000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Escherichia coli
glyoxylate cycle
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
4.1.3.1
4°C
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33115
Escherichia coli
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33115
Escherichia coli
succinate + glyoxylate
-
33115
Escherichia coli
r
4.1.3.1
isocitrate
glyoxylate cycle
33115
Escherichia coli
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 48000, SDS-PAGE
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.3
-
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.008
-
threo-Ds-isocitrate
-
Escherichia coli
4.1.3.1
0.015
-
DL-isocitrate
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
48000
-
4 * 48000, SDS-PAGE
Escherichia coli
4.1.3.1
188000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Escherichia coli
glyoxylate cycle
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.3.1
4°C
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33115
Escherichia coli
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33115
Escherichia coli
succinate + glyoxylate
-
33115
Escherichia coli
r
4.1.3.1
isocitrate
glyoxylate cycle
33115
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 48000, SDS-PAGE
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.3
-
-
Escherichia coli