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Literature summary extracted from

  • Löffler, F.; Lingens, F.; Muller, R.
    Dehalogenation of 4-chlorobenzoate. Characterisation of 4-chlorobenzoyl-coenzyme A dehalogenase from Pseudomonas sp. CBS3 (1995), Biodegradation, 6, 203-212.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.8.1.7 pure enzyme is completely inactive after a single freeze and thawing cycle Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.8.1.7 5,5'-dithiobis(2-nitrobenzoate)
-
 Pseudomonas sp.
3.8.1.7 Ag+ 5 mM Pseudomonas sp.
3.8.1.7 Co2+ 5 mM Pseudomonas sp.
3.8.1.7 Cu2+ 5 mM Pseudomonas sp.
3.8.1.7 Fe2+ 5 mM Pseudomonas sp.
3.8.1.7 Mn2+ 5 mM Pseudomonas sp.
3.8.1.7 Ni2+ 5 mM Pseudomonas sp.
3.8.1.7 SDS 1 mM, 80% inhibition Pseudomonas sp.
3.8.1.7 Zn2+ 5 mM Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.7 0.0024 0.0027 4-chlorobenzoyl-CoA
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.7 12000
-
 gel filtration Pseudomonas sp.
3.8.1.7 31000
-
 4 * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE Pseudomonas sp.
3.8.1.7 31000
-
 8 * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE Pseudomonas sp.
3.8.1.7 31000
-
 x * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.7 Pseudomonas sp.
-
-
-
3.8.1.7 Pseudomonas sp. CBS-3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.7
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.7 2.25
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
3.8.1.7 -20°C, impure preparations, stable for several months Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.7 4-bromobenzoyl-CoA + H2O
-
 Pseudomonas sp. 4-hydroxybenzoyl-CoA + bromide
-
 ?
3.8.1.7 4-bromobenzoyl-CoA + H2O
-
 Pseudomonas sp. CBS-3 4-hydroxybenzoyl-CoA + bromide
-
 ?
3.8.1.7 4-chlorobenzoyl-CoA + H2O
-
 Pseudomonas sp. 4-hydroxybenzoyl-CoA + chloride
-
 ?
3.8.1.7 4-chlorobenzoyl-CoA + H2O
-
 Pseudomonas sp. CBS-3 4-hydroxybenzoyl-CoA + chloride
-
 ?
3.8.1.7 4-fluorobenzoyl-CoA + H2O no activity Pseudomonas sp. 4-hydroxybenzoyl-CoA + fluoride
-
 ?
3.8.1.7 4-fluorobenzoyl-CoA + H2O no activity Pseudomonas sp. CBS-3 4-hydroxybenzoyl-CoA + fluoride
-
 ?
3.8.1.7 4-iodobenzoyl-CoA + H2O
-
 Pseudomonas sp. 4-hydroxybenzoyl-CoA + iodide
-
 ?
3.8.1.7 4-iodobenzoyl-CoA + H2O
-
 Pseudomonas sp. CBS-3 4-hydroxybenzoyl-CoA + iodide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.7 multimer x * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE Pseudomonas sp.
3.8.1.7 octamer 8 * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE Pseudomonas sp.
3.8.1.7 tetramer 4 * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.7 60
-
-
 Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.8.1.7 30 65 about 60% of maximal activity at 30°C and at 65°C Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.8.1.7 50
-
 15 min, stable Pseudomonas sp.
3.8.1.7 60
-
 15 min, 30% loss of activity Pseudomonas sp.
3.8.1.7 65
-
 15 min, complete loss of activity Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8.1.7 5
-
 4-chlorobenzoyl-CoA
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.7 10
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.8.1.7 9 11.5 about 50% of maximal activity at pH 9.0 and at pH 11.5 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.8.1.7 4.5
-
 10 min, complete loss of activity Pseudomonas sp.
3.8.1.7 6
-
 60 min, 10% loss of activity Pseudomonas sp.
3.8.1.7 6.5 11 60 min, stable Pseudomonas sp.
3.8.1.7 11.5
-
 60 min, 10% loss of activity Pseudomonas sp.
3.8.1.7 12
-
 10 min, complete loss of activity Pseudomonas sp.