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Literature summary extracted from
- Subcloning of the enterobactin biosynthetic gene entB: expression, purification, characterization, and substrate specificity of isochorismatase (1990), Biochemistry, 29, 1425-1435.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.3.2.1 | - |
Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.3.2.1 | (+/-)-3-[(carboxyethenyl)oxy]-6-hydroxy-1-benzoic acid | i.e. 5-(1-carboxyvinyloxy)-2-hydroxybenzoic acid | Escherichia coli |  |
| 3.3.2.1 | 3-[(carboxyethenyl)oxy]benzoic acid | i.e. 3-(1-carboxyvinyloxy)benzoic acid | Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.3.2.1 | 0.015 | - |
isochorismate | - |
Escherichia coli | |
| 3.3.2.1 | 0.023 | - |
(+/-)-3-[(1-carboxyethenyl)oxy]-2-hydroxy-6-cyclohexene-1-carboxylic acid | - |
Escherichia coli | |
| 3.3.2.1 | 0.086 | - |
(+/-)-3-[(carboxyethenyl)oxy]-1-cyclohexene-1-carboxylic acid | - |
Escherichia coli | |
| 3.3.2.1 | 0.12 | - |
(+/-)-3-[(1-carboxyethenyl)oxy]-cyclohepta-1,6-diene-1-carboxylate | - |
Escherichia coli | |
| 3.3.2.1 | 0.28 | - |
(+/-)-cis-3-[(carboxyethenyl)oxy]-4-cyclohexene-1-carboxylic acid | - |
Escherichia coli | |
| 3.3.2.1 | 35 | - |
chorismate | - |
Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.3.2.1 | 35000 | - |
5 * 35000, SDS-PAGE | Escherichia coli |
| 3.3.2.1 | 159000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.3.2.1 | isochorismate + H2O | Escherichia coli | biosynthesis of enterobactin | 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.3.2.1 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.3.2.1 | - |
Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.3.2.1 | -20°C, 25 mM Tris buffer, pH 8.0, 5 mM dithiothreitol, 50% glycerol, stable for several months | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.3.2.1 | (+/-)-3-[(1-carboxyethenyl)oxy]-2-hydroxy-6-cyclohexene-1-carboxylic acid + H2O | - |
Escherichia coli | 3,4-dihydroxy-cyclohex-1-enecarboxylic acid + pyruvate | - |
? | |
| 3.3.2.1 | (+/-)-3-[(1-carboxyethenyl)oxy]-cyclohepta-1,6-diene-1-carboxylate + H2O | i.e. (S)-3-(1-carboxyvinyloxy)-cyclohepta-1,6-dienecarboxylic acid | Escherichia coli | (S)-3-hydroxy-cyclohepta-1,6-dienecarboxylic acid + pyruvate | - |
? | |
| 3.3.2.1 | (+/-)-3-[(carboxyethenyl)oxy]-1-cyclohexene-1-carboxylic acid + H2O | - |
Escherichia coli | (S)-3-hydroxycyclohex-1-enecarboxylic acid + pyruvate | - |
? | |
| 3.3.2.1 | (+/-)-cis-3-[(carboxyethenyl)oxy]-4-cyclohexene-1-carboxylic acid + H2O | - |
Escherichia coli | (R)-5-hydroxycyclohex-3-enecarboxylic acid + pyruvate | - |
? | |
| 3.3.2.1 | chorismate + H2O | slow hydrolysis | Escherichia coli | 3,4-dihydroxy-cyclohexa-1,5-dienecarboxylic acid + pyruvate | - |
? | |
| 3.3.2.1 | isochorismate + H2O | - |
Escherichia coli | 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate | - |
? | |
| 3.3.2.1 | isochorismate + H2O | biosynthesis of enterobactin | Escherichia coli | 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.3.2.1 | pentamer | 5 * 35000, SDS-PAGE | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.3.2.1 | 5.17 | - |
(+/-)-3-[(1-carboxyethenyl)oxy]-2-hydroxy-6-cyclohexene-1-carboxylic acid | - |
Escherichia coli | |
| 3.3.2.1 | 9 | - |
(+/-)-3-[(1-carboxyethenyl)oxy]-cyclohepta-1,6-diene-1-carboxylate | - |
Escherichia coli | |
| 3.3.2.1 | 10 | - |
isochorismate | - |
Escherichia coli | |
| 3.3.2.1 | 16.3 | - |
(+/-)-cis-3-[(carboxyethenyl)oxy]-4-cyclohexene-1-carboxylic acid | - |
Escherichia coli | |
| 3.3.2.1 | 21.7 | - |
(+/-)-3-[(carboxyethenyl)oxy]-1-cyclohexene-1-carboxylic acid | - |
Escherichia coli | |
| 3.3.2.1 | 217 | - |
chorismate | - |
Escherichia coli | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.3.2.1 | 6.5 | 7.5 | - |
Escherichia coli |
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