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Literature summary extracted from
- Regulation of the enzymes involved in the biosynthesis of 2,3-dihydroxybenzoic acid in Aerobacter aerogenes and Escherichia coli (1969), Biochim. Biophys. Acta, 177, 401-411.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.3.1.28 | unstable after chromatography on DEAE cellulose | Klebsiella aerogenes |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.3.2.1 | Fe2+ | - |
Klebsiella aerogenes |  |
| 5.4.4.2 | EDTA | - |
Klebsiella aerogenes | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.4.2 | Mg2+ | stimulates, optimal concentration: 2-5 mM | Klebsiella aerogenes | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.3.2.1 | isochorismate + H2O | Klebsiella aerogenes | - |
2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.1.28 | Klebsiella aerogenes | - |
- |
- |
| 3.3.2.1 | Klebsiella aerogenes | - |
- |
- |
| 5.4.4.2 | Klebsiella aerogenes | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.28 | 0.0046 | - |
enzyme activity in crude extracts of cells grown in the presence of 0.002 mM iron, enzyme is repressed by high iron concentrations | Klebsiella aerogenes |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.3.2.1 | isochorismate + H2O | - |
Klebsiella aerogenes | 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate | - |
? | |
| 5.4.4.2 | Chorismate | r | Klebsiella aerogenes | Isochorismate | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.4.2 | 8 | - |
- |
Klebsiella aerogenes |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.4.2 | 5.5 | 8.5 | pH 5.5: about 20% of maximal activity, pH 8.5: about 90% of maximal activity | Klebsiella aerogenes |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.28 | NAD+ | absolutely required | Klebsiella aerogenes | |
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Release 2023.1 (January 2023)
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