BRENDA - Enzyme Database show

NAD(P)+-glycohydrolase from human spleen: a multicatalytic enzyme

Orsomando, G.; Polzonetti, V.; Natalini, P.; Comp. Biochem. Physiol. B 126, 89-98 (2000)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.2.2.6
50% inactivation at 2 M urea
Homo sapiens
3.2.2.6
SDS, even at micromolar concentration, leads to complete inactivation of the enzyme
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.6
Ag+
50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase; at 2 mM 50% inhibition of NADase activity and 300% activation of cADPR activity
Homo sapiens
3.2.2.6
Cr3+
; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
3.2.2.6
Hg2+
complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
3.2.2.6
Pb2+
complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
3.2.2.6
SDS
-
Homo sapiens
3.2.2.6
Sn2+
complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
3.2.2.6
Zn2+
; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.6
0.026
-
NAD+
; pH 7.0, 28°C
Homo sapiens
3.2.2.6
0.033
-
epsilonNAD+
-
Homo sapiens
3.2.2.6
0.033
-
nicotinamide 1,N6-ethenoadenine dinucleotide
pH 7.0, 28°C
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.2.6
membrane
-
Homo sapiens
16020
-
3.2.2.6
microsome
-
Homo sapiens
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.2.6
Ag+
50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase; at 2 mM 50% inhibition of NADase activity and 300% activation of ADPR activity
Homo sapiens
3.2.2.6
Cu2+
stimulation of cADPR activity
Homo sapiens
3.2.2.6
additional information
no effects by K+, Cd2+, Fe2+, Fe3+, and Mn2+
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.2.6
additional information
-
native enzyme shows high molecular weight and elutes in the void volumes from a Superose 12 gel filtration column
Homo sapiens
3.2.2.6
45000
-
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.6
additional information
Homo sapiens
enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.2.6
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.2.6
partial; partially from spleen microsomal membranes after solubilization with Triton X-100, chromatography methods, 133fold
Homo sapiens
Reaction
EC Number
Reaction
Commentary
Organism
3.2.2.6
NAD+ + H2O = ADP-D-ribose + nicotinamide
also catalyses transfer of ADP-ribose(P) residues
Homo sapiens
Renatured (Commentary)
EC Number
Commentary
Organism
3.2.2.6
80% renaturation of urea and SDS denatured enzyme by treatment with Triton X-100 in a 20:1 v/w ratio with SDS
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.2.6
spleen
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.2.6
0.02
-
partially purified enzyme, ADPR activity
Homo sapiens
3.2.2.6
0.113
-
partially purified enzyme, cADPR activity
Homo sapiens
3.2.2.6
2.205
-
partially purified enzyme, NADase activity
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
3.2.2.6
20°C, lyophilized enzyme, 90% remaining activity after 6 months
Homo sapiens
3.2.2.6
4°C to -196°C, 10 mM Tris-HCl, pH 8.0, 0.1% Triton X-100, at least 1 month, stable
Homo sapiens
3.2.2.6
4°C, -20°C, -80°C or -196°C, pH 8.0, 0.1% Triton X-100, 10 mM Tris-HCl, stable for at least 1 month
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.6
3-acetylpyridine + adenosine diphosphoribose
transglycosylation activity, very poor hydrolytic activity with this substrate
326409
Homo sapiens
3-acetylpyridine adenine dinucleotide + phosphate
-
-
-
?
3.2.2.6
3-acetylpyridine adenine dinucleotide + H2O
-
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
3-acetylpyridine hypoxanthine dinucleotide + H2O
low activity
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
3-pyridine adenine dinucleotide + H2O
-
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
3-pyridinealdehyde hypoxanthine dinucleotide + H2O
-
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
3-pyridinealdehyde hypoxanthine dinucleotide + H2O
60% activity compared to the activity with NAD+
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
cyclic ADP-ribose + H2O
-
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
cyclic ADP-ribose + H2O
i.e. ADPR activity
326409
Homo sapiens
adenosine diphosphoribose + ?
-
-
-
?
3.2.2.6
epsilon-NAD+ + H2O
-
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
epsilon-NADP+ + H2O
-
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
additional information
enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose
326409
Homo sapiens
?
-
-
-
-
3.2.2.6
additional information
enzyme is multicatalytic, it also catalyzes the synthesis of cyclic ADP-ribose from NAD+ and the hydrolysis to adenosine diphosphoribose
326409
Homo sapiens
?
-
-
-
-
3.2.2.6
NAD+
-
326409
Homo sapiens
cyclic ADP-ribose + nicotinamide
-
326409
Homo sapiens
?
3.2.2.6
NAD+ + ?
i.e. cADPR activity
326409
Homo sapiens
cyclic ADP-ribose + ?
-
-
-
?
3.2.2.6
NAD+ + H2O
-
326409
Homo sapiens
ADP-ribose + nicotinamide
-
-
-
?
3.2.2.6
NADP+ + H2O
-
326409
Homo sapiens
nicotinamide + ADPribose-phosphate
-
-
-
?
3.2.2.6
NADP+ + H2O
80% activity compared to the activity with NAD+
326409
Homo sapiens
ADP-ribose-P + nicotinamide
-
-
-
?
3.2.2.6
NGD+ + H2O
as effective as substrate as NAD+
326409
Homo sapiens
GDP-ribose + nicotinamide
-
-
-
?
3.2.2.6
nicotinamide 1,N6-ethenoadenine dinucleotide + H2O
i.e. epsilonNAD+, used for a fluorometric assay
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
nicotinamide guanine dinucleotide + H2O
-
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
nicotinamide-hypoxanthine dinucleotide + H2O
-
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
thio-NAD+ + H2O
-
326409
Homo sapiens
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.2.6
oligomer
x * 45000, SDS-PAGE
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.2.6
38
-
-
Homo sapiens
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.2.2.6
38
50
inactive above 50°C
Homo sapiens
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.2.6
38
-
half-life: about 1 day
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.6
7
-
; NADase activity
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.2.6
4.5
9.5
-
Homo sapiens
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.2.2.6
Homo sapiens
isoelectric focusing
-
4.9
General Stability (protein specific)
EC Number
General Stability
Organism
3.2.2.6
50% inactivation at 2 M urea
Homo sapiens
3.2.2.6
SDS, even at micromolar concentration, leads to complete inactivation of the enzyme
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.6
Ag+
50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase; at 2 mM 50% inhibition of NADase activity and 300% activation of cADPR activity
Homo sapiens
3.2.2.6
Cr3+
; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
3.2.2.6
Hg2+
complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
3.2.2.6
Pb2+
complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
3.2.2.6
SDS
-
Homo sapiens
3.2.2.6
Sn2+
complete inhibition at 2 mM; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
3.2.2.6
Zn2+
; inhibition of NADase and adenosine diphosphate cyclase
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.6
0.026
-
NAD+
; pH 7.0, 28°C
Homo sapiens
3.2.2.6
0.033
-
epsilonNAD+
-
Homo sapiens
3.2.2.6
0.033
-
nicotinamide 1,N6-ethenoadenine dinucleotide
pH 7.0, 28°C
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.2.6
membrane
-
Homo sapiens
16020
-
3.2.2.6
microsome
-
Homo sapiens
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.2.6
Ag+
50% inhibition of NADase and 300% activation of adenosine diphosphate cyclase; at 2 mM 50% inhibition of NADase activity and 300% activation of ADPR activity
Homo sapiens
3.2.2.6
Cu2+
stimulation of cADPR activity
Homo sapiens
3.2.2.6
additional information
no effects by K+, Cd2+, Fe2+, Fe3+, and Mn2+
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.2.6
additional information
-
native enzyme shows high molecular weight and elutes in the void volumes from a Superose 12 gel filtration column
Homo sapiens
3.2.2.6
45000
-
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.6
additional information
Homo sapiens
enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.2.6
partial; partially from spleen microsomal membranes after solubilization with Triton X-100, chromatography methods, 133fold
Homo sapiens
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.2.6
80% renaturation of urea and SDS denatured enzyme by treatment with Triton X-100 in a 20:1 v/w ratio with SDS
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.2.6
spleen
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.2.6
0.02
-
partially purified enzyme, ADPR activity
Homo sapiens
3.2.2.6
0.113
-
partially purified enzyme, cADPR activity
Homo sapiens
3.2.2.6
2.205
-
partially purified enzyme, NADase activity
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.2.6
20°C, lyophilized enzyme, 90% remaining activity after 6 months
Homo sapiens
3.2.2.6
4°C to -196°C, 10 mM Tris-HCl, pH 8.0, 0.1% Triton X-100, at least 1 month, stable
Homo sapiens
3.2.2.6
4°C, -20°C, -80°C or -196°C, pH 8.0, 0.1% Triton X-100, 10 mM Tris-HCl, stable for at least 1 month
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.6
3-acetylpyridine + adenosine diphosphoribose
transglycosylation activity, very poor hydrolytic activity with this substrate
326409
Homo sapiens
3-acetylpyridine adenine dinucleotide + phosphate
-
-
-
?
3.2.2.6
3-acetylpyridine adenine dinucleotide + H2O
-
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
3-acetylpyridine hypoxanthine dinucleotide + H2O
low activity
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
3-pyridine adenine dinucleotide + H2O
-
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
3-pyridinealdehyde hypoxanthine dinucleotide + H2O
-
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
3-pyridinealdehyde hypoxanthine dinucleotide + H2O
60% activity compared to the activity with NAD+
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
cyclic ADP-ribose + H2O
-
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
cyclic ADP-ribose + H2O
i.e. ADPR activity
326409
Homo sapiens
adenosine diphosphoribose + ?
-
-
-
?
3.2.2.6
epsilon-NAD+ + H2O
-
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
epsilon-NADP+ + H2O
-
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
additional information
enzyme is involved in the regulation of intracellular concentration of adenosine diphosphoribose
326409
Homo sapiens
?
-
-
-
-
3.2.2.6
additional information
enzyme is multicatalytic, it also catalyzes the synthesis of cyclic ADP-ribose from NAD+ and the hydrolysis to adenosine diphosphoribose
326409
Homo sapiens
?
-
-
-
-
3.2.2.6
NAD+
-
326409
Homo sapiens
cyclic ADP-ribose + nicotinamide
-
326409
Homo sapiens
?
3.2.2.6
NAD+ + ?
i.e. cADPR activity
326409
Homo sapiens
cyclic ADP-ribose + ?
-
-
-
?
3.2.2.6
NAD+ + H2O
-
326409
Homo sapiens
ADP-ribose + nicotinamide
-
-
-
?
3.2.2.6
NADP+ + H2O
-
326409
Homo sapiens
nicotinamide + ADPribose-phosphate
-
-
-
?
3.2.2.6
NADP+ + H2O
80% activity compared to the activity with NAD+
326409
Homo sapiens
ADP-ribose-P + nicotinamide
-
-
-
?
3.2.2.6
NGD+ + H2O
as effective as substrate as NAD+
326409
Homo sapiens
GDP-ribose + nicotinamide
-
-
-
?
3.2.2.6
nicotinamide 1,N6-ethenoadenine dinucleotide + H2O
i.e. epsilonNAD+, used for a fluorometric assay
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
nicotinamide guanine dinucleotide + H2O
-
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
nicotinamide-hypoxanthine dinucleotide + H2O
-
326409
Homo sapiens
?
-
-
-
?
3.2.2.6
thio-NAD+ + H2O
-
326409
Homo sapiens
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.2.6
oligomer
x * 45000, SDS-PAGE
Homo sapiens
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.2.6
38
-
-
Homo sapiens
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.2.2.6
38
50
inactive above 50°C
Homo sapiens
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.2.6
38
-
half-life: about 1 day
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.6
7
-
; NADase activity
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.2.6
4.5
9.5
-
Homo sapiens
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.2.2.6
Homo sapiens
isoelectric focusing
-
4.9