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Literature summary extracted from

  • Mather, I.H.; Knight, M.
    A heat-stable nicotinamide-adenine dinucleotide glycohydrolase from Pseudomonas putida KB1. Partial purification and some properties of the enzyme and an inhibitory protein (1972), Biochem. J., 129, 141-152.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.6 additional information NADase inhibitor from Pseudomonas putida KB1 Pseudomonas putida KB1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.6 0.25
-
 thio-NAD+
-
 Pseudomonas putida KB1
3.2.2.6 0.51
-
 nicotinamide-hypoxanthine dinucleotide phosphate
-
 Pseudomonas putida KB1
3.2.2.6 0.66
-
 NADP+
-
 Pseudomonas putida KB1
3.2.2.6 0.85
-
 NAD+
-
 Pseudomonas putida KB1
3.2.2.6 1.44
-
 nicotinamide-hypoxanthine dinucleotide
-
 Pseudomonas putida KB1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.6 23500
-
 gel filtration Pseudomonas putida KB1

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.6 Pseudomonas putida KB1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.6 partial Pseudomonas putida KB1

Storage Stability

EC Number Storage Stability Organism
3.2.2.6 -20°C, stable for several weeks Pseudomonas putida KB1
3.2.2.6 4°C, 0.1 M Tris-HCl buffer, pH 7.0, 50% loss of activity Pseudomonas putida KB1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.6 beta-NAD+ + H2O beta-NAD+ and NADP+ are hydrolyzed at equal rates Pseudomonas putida KB1 ?
-
 ?
3.2.2.6 NADP+ + H2O
-
 Pseudomonas putida KB1 nicotinamide + ADPribose-phosphate
-
 ?
3.2.2.6 nicotinamide-hypoxanthine dinucleotide + H2O
-
 Pseudomonas putida KB1 ?
-
 ?
3.2.2.6 nicotinamide-hypoxanthine dinucleotide phosphate + H2O
-
 Pseudomonas putida KB1 ?
-
 ?
3.2.2.6 thio-NAD+ + H2O
-
 Pseudomonas putida KB1 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.6 40
-
-
 Pseudomonas putida KB1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.2.6 100
-
 60% loss of activity after 30 min, 80% loss of activity after 1 h Pseudomonas putida KB1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.6 5 10 citrate buffer, activity is essentially unchanged Pseudomonas putida KB1
3.2.2.6 6 10 phosphate buffer, activity is essentially unchanged Pseudomonas putida KB1

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.2.6 1 10 5 min, 50 mM citrate, stable Pseudomonas putida KB1
3.2.2.6 4 5 5 min, less than 10% loss of activity Pseudomonas putida KB1