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Literature summary extracted from

  • Parkin, D.W.; Horenstein, B.A.; Abdulah, D.R.; Estupinan, B.; Schramm, V.L.
    Nucleoside hydrolase from Crithidia fasciculata. Metabolic role, purification, specificity, and kinetic mechanism (1991), J. Biol. Chem., 266, 20658-20665.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.1 7-deazaadenosine i.e. tubercidin Crithidia fasciculata
3.2.2.1 D-ribose product inhibition Crithidia fasciculata
3.2.2.1 hypoxanthine product inhibition Crithidia fasciculata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.1 0.145
-
 purine riboside
-
 Crithidia fasciculata
3.2.2.1 0.38
-
 Inosine
-
 Crithidia fasciculata
3.2.2.1 0.42
-
 guanosine
-
 Crithidia fasciculata
3.2.2.1 0.46
-
 adenosine
-
 Crithidia fasciculata
3.2.2.1 1.22
-
 uridine
-
 Crithidia fasciculata
3.2.2.1 1.3
-
 5-methyluridine
-
 Crithidia fasciculata
3.2.2.1 4.7
-
 cytosine
-
 Crithidia fasciculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.1 34000
-
 4 * 34000, SDS-PAGE Crithidia fasciculata
3.2.2.1 143000
-
 gel filtration Crithidia fasciculata

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.1 Crithidia fasciculata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.1
-
 Crithidia fasciculata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.1 0.047
-
-
 Crithidia fasciculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.1 5-methyluridine + H2O
-
 Crithidia fasciculata 5-methyluracil + D-ribose
-
 ?
3.2.2.1 adenosine + H2O
-
 Crithidia fasciculata D-ribose + adenine
-
 ?
3.2.2.1 cytosine + D-ribose
-
 Crithidia fasciculata cytidine + H2O
-
 r
3.2.2.1 guanosine + H2O
-
 Crithidia fasciculata D-ribose + guanine
-
 ?
3.2.2.1 inosine + H2O kinetic mechanism is rapid equilibrium with random product release Crithidia fasciculata hypoxanthine + D-ribose
-
 ?
3.2.2.1 purine ribonucleoside + H2O
-
 Crithidia fasciculata purine + D-ribose
-
 ?
3.2.2.1 purine riboside + H2O
-
 Crithidia fasciculata purine + D-ribose
-
 ?
3.2.2.1 uridine + H2O
-
 Crithidia fasciculata D-ribose + uracil
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.1 tetramer 4 * 34000, SDS-PAGE Crithidia fasciculata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.1 32
-
 Inosine
-
 Crithidia fasciculata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.2.1 5.5 9.7 stable Crithidia fasciculata