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Literature summary extracted from

  • Yoshioka, H.; Hayashida, S.
    Purification and properties of beta-glucosidase from Humicola insolvens YH-8 (1980), Agric. Biol. Chem., 44, 1729-1735.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.21 stable in the presence of dimethyl sulfoxide or ethyl alcohol Humicola insolens
3.2.1.21 stable in the presence of urea Humicola insolens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Cu2+ 55% of original activity inhibitited Humicola insolens
3.2.1.21 NaCl low activity even at low concentrations Humicola insolens
3.2.1.21 Sr2+ slight inhibition at 10 mM Humicola insolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.53
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Humicola insolens
3.2.1.21 0.86
-
 Salicin
-
 Humicola insolens
3.2.1.21 1.52
-
 cellobiose
-
 Humicola insolens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 extracellular
-
 Humicola insolens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 250000
-
 SDS-PAGE Humicola insolens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Humicola insolens cellobiose beta-D-glucose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Humicola insolens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.21 glycoprotein 1.7%, w/w, mannose, 0.8%, w/w, glucose residues Humicola insolens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, chromatography techniques Humicola insolens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture maximal enzyme synthesis, if cells grown in wheat bran medium Humicola insolens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 0.05
-
-
 Humicola insolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Humicola insolens 2-hydroxymethylphenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Humicola insolens 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Humicola insolens beta-D-glucose + D-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 50
-
-
 Humicola insolens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 60
-
 stable for 5 min Humicola insolens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5
-
-
 Humicola insolens