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Literature summary extracted from

  • Shewale, J.G.; Sadana, J.
    Purification, characterization, and properties of beta-glucosidase enzymes from Sclerotium rolfsii (1981), Arch. Biochem. Biophys., 207, 185-196.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 beta-D-glucose Ki: 0.55 mM Athelia rolfsii
3.2.1.21 D-glucono-1,5-lactone Ki: 0.01 mM Athelia rolfsii
3.2.1.21 nojirimycin bisulfite Ki: 0.001 mM Athelia rolfsii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.79 1.38 4-nitrophenyl-beta-D-glucopyranoside 4 different isoforms Athelia rolfsii
3.2.1.21 3.07 5.84 cellobiose 4 different isoforms, Km depends on pH-value, ionizing groups control binding of substrate Athelia rolfsii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 extracellular
-
 Athelia rolfsii
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 90000
-
 beta-glucosidases 1 and 2, gel filtration Athelia rolfsii
3.2.1.21 107000
-
 beta-glucosidase 3, gel filtration Athelia rolfsii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Athelia rolfsii cellobiose beta-D-glucose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Athelia rolfsii
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.21 glycoprotein contains alpha-D-mannose-, alpha-D-glucose- and 2-O-D-mannose-residues Athelia rolfsii

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, 4 different forms, chromatography steps Athelia rolfsii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture 4 different isoforms Athelia rolfsii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 51 175 4 different isoforms, cellobiose as substrate Athelia rolfsii

Storage Stability

EC Number Storage Stability Organism
3.2.1.21 -15°C, pH 4.5, 12 months Athelia rolfsii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Athelia rolfsii 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Athelia rolfsii beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Athelia rolfsii 2 beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 90000-107000, SDS-PAGE, 4 isoforms Athelia rolfsii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 65 68
-
 Athelia rolfsii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 70
-
 50% of activity remains after 10 min Athelia rolfsii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 4.2 4.5 depending on substrate used Athelia rolfsii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 4 4.5 most stable within Athelia rolfsii