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Literature summary extracted from

  • Ferreira, C.; Terra, W.R.
    Physical and kinetic properties of a plasma-membrane-bound beta-D-glucosidase (cellobiase) from midgut cells of an insect (Rhynchosciara americana larva) (1983), Biochem. J., 213, 43-51.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 4-nitrophenyl-alpha-D-glucoside 48.6% inhibition at 15 mM Rhynchosciara americana
3.2.1.21 D-glucono-1,5-lactone competitive inhibition, Ki: 0.0005 mM Rhynchosciara americana
3.2.1.21 gamma-galactonolactone competitive inhibition, Ki: 0.3 mM Rhynchosciara americana
3.2.1.21 maltose 44.8% inhibition at 15 mM Rhynchosciara americana
3.2.1.21 melibiose 20.2% inhibition at 15 mM Rhynchosciara americana
3.2.1.21 sucrose 51% inhibition at 15 mM Rhynchosciara americana
3.2.1.21 Tris competitive inhibition, Ki: 19 mM Rhynchosciara americana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.5
-
 4-nitrophenyl-beta-D-fucoside
-
 Rhynchosciara americana
3.2.1.21 0.53
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Rhynchosciara americana
3.2.1.21 0.61
-
 cellobiose
-
 Rhynchosciara americana
3.2.1.21 0.8
-
 Salicin
-
 Rhynchosciara americana
3.2.1.21 0.9
-
 2-Nitrophenyl-beta-D-glucopyranoside
-
 Rhynchosciara americana
3.2.1.21 3.5
-
 phenyl-beta-D-glucoside
-
 Rhynchosciara americana
3.2.1.21 5.3
-
 4-nitrophenyl-beta-D-galactoside
-
 Rhynchosciara americana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 plasma membrane detergent-solubilized and papain-released forms Rhynchosciara americana 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 106000
-
 detergent-solubilized form, density-gradient centrifugation Rhynchosciara americana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Rhynchosciara americana cellobiose beta-D-glucose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Rhynchosciara americana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 near homogeneity, density gradient centrifugation Rhynchosciara americana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 midgut from larvae Rhynchosciara americana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O low activity Rhynchosciara americana 2-hydroxymethylphenol + beta-D-glucose
-
 ?
3.2.1.21 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Rhynchosciara americana 2-hydroxymethylphenol + beta-D-glucose
-
 ?
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O retention of configuration Rhynchosciara americana 2-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-fucoside + H2O
-
 Rhynchosciara americana 4-nitrophenol + beta-D-fucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactoside + H2O
-
 Rhynchosciara americana 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Rhynchosciara americana 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Rhynchosciara americana beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Rhynchosciara americana 2 beta-D-glucose
-
 ?
3.2.1.21 phenyl-beta-D-glucoside + H2O low activity Rhynchosciara americana phenol + beta-D-glucose
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 40
-
 completely stable up to for 2 h Rhynchosciara americana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6.2
-
-
 Rhynchosciara americana