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Literature summary extracted from

  • Araujo, E.F.; Barros, E.G.; Caldas, R.A.; Silva, D.O.
    beta-Glucosidase activity of thermophilic cellulolytic fungus, Humicola sp. (1983), Biotechnol. Lett., 5, 781-784.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.21 storage at -13°C, 4°C or 25°C for 71 days: extracellular enzyme unaffected Humicola sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 4-nitrophenyl-beta-D-glucopyranoside inhibition of intracellular enzyme if higher than 0.4 mM Humicola sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.135
-
 4-nitrophenyl-beta-glucopyranoside extracellular enzyme Humicola sp.
3.2.1.21 0.41
-
 4-nitrophenyl-beta-D-glucopyranoside intracellular enzyme Humicola sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 extracellular
-
 Humicola sp.
-
-
3.2.1.21 intracellular
-
 Humicola sp. 5622
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Humicola sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture
-
 Humicola sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Humicola sp. 4-nitrophenol + D-glucopyranose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 55
-
 intracellular enzyme Humicola sp.
3.2.1.21 75
-
 extracellular enzyme Humicola sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 60
-
 extracellular enzyme: 25% loss of activity in 1 h, intracellular enzyme: 80% loss of activity in 1 h Humicola sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5
-
 intra- and extracellular enzymes Humicola sp.