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Literature summary extracted from

  • Srivastava, S.K.; Gopalkrishnan, K.S.; Ramachandran, K.B.
    Kinetic characterization of a crude beta-D-glucosidase from Aspergillus wentii Pt 2804 (1984), Enzyme Microb. Technol., 6, 508-512.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 beta-D-glucose competitive inhibition, Ki: 14 mM Aspergillus wentii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 1.6
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Aspergillus wentii
3.2.1.21 8
-
 cellobiose
-
 Aspergillus wentii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Aspergillus wentii cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Aspergillus wentii synergistic action of Trichoderma reesei and Aspergillus wentii cellulases for cellulose hydrolysis beta-D-glucose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Aspergillus wentii
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture
-
 Aspergillus wentii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus wentii 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Aspergillus wentii beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O synergistic action of Trichoderma reesei and Aspergillus wentii cellulases for cellulose hydrolysis Aspergillus wentii beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Aspergillus wentii 2 beta-D-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 60
-
 hydrolysis of 4-nitrophenyl-beta-D-glucopyranoside Aspergillus wentii
3.2.1.21 65
-
 hydrolysis of cellobiose Aspergillus wentii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 60
-
 half-life: 38 h Aspergillus wentii
3.2.1.21 65
-
 half-life: 6.3 h Aspergillus wentii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 3.5 5 hydrolysis of 2-nitrophenyl-beta-D-glucopyranoside Aspergillus wentii
3.2.1.21 4.5 5.5 hydrolysis of cellobiose Aspergillus wentii