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Literature summary extracted from

  • Beldman, G.; Searle-Van Leeuwen, M.F.; Rombouts, F.M.; Voragen, F.G.J.
    The cellulase of Trichoderma viride. Purification, characterization and comparison of all detectable endoglucanases, exoglucanases and beta-glucosidases (1985), Eur. J. Biochem., 146, 301-308.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 23500
-
 x * 23500, endoglucanase IV, SDS-PAGE Trichoderma viride
3.2.1.4 45000
-
 x * 45000, endoglucanase II, SDS-PAGE Trichoderma viride
3.2.1.4 50000
-
 x * 50000, endoglucanase I, SDS-PAGE Trichoderma viride
3.2.1.4 52000
-
 x * 52000, endoglucanase VI, SDS-PAGE Trichoderma viride
3.2.1.4 57000
-
 x * 57000, endoglucanase V, SDS-PAGE Trichoderma viride
3.2.1.4 585000
-
 x * 585000, endoglucanase III, SDS-PAGE Trichoderma viride
3.2.1.21 76000
-
 SDS-PAGE Trichoderma viride

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 cellulose + H2O Trichoderma viride H3PO4-swollen beta-D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Trichoderma viride
-
-
-
3.2.1.21 Trichoderma viride
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.4 glycoprotein endoglucanases I, II, III, V, and VI are glycoproteins Trichoderma viride
3.2.1.4 no modification endoglucanase IV contains no carbohydrate Trichoderma viride

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 endoglucanase I, II, III, IV, V and VI Trichoderma viride
3.2.1.21 to homogeneity, further preparation of commercial cellulase preparation Trichoderma viride

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 commercial preparation Maxazyme Cl Trichoderma viride
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Trichoderma viride 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 Avicel + H2O moderately active Trichoderma viride beta-D-glucose + ?
-
 ?
3.2.1.21 carboxymethyl-cellulose + H2O low activity Trichoderma viride beta-D-glucose + ?
-
 ?
3.2.1.21 cellulose + H2O H3PO4-swollen Trichoderma viride beta-D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 45000, endoglucanase II, SDS-PAGE Trichoderma viride
3.2.1.4 ? x * 52000, endoglucanase VI, SDS-PAGE Trichoderma viride
3.2.1.4 ? x * 57000, endoglucanase V, SDS-PAGE Trichoderma viride
3.2.1.4 ? x * 585000, endoglucanase III, SDS-PAGE Trichoderma viride
3.2.1.4 ? x * 50000, endoglucanase I, SDS-PAGE Trichoderma viride
3.2.1.4 ? x * 23500, endoglucanase IV, SDS-PAGE Trichoderma viride

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 48
-
 endoglucanase IV Trichoderma viride
3.2.1.4 50
-
 endoglucanase V and VI Trichoderma viride
3.2.1.4 60
-
 endoglucanase I, II, and III Trichoderma viride
3.2.1.21 52
-
-
 Trichoderma viride