Any feedback?
Literature summary extracted from
- beta-Glucosidase of Penicillium funiculosum. II: Properties and mycelial binding (1985), Biotechnol. Bioeng., 23, 786-791.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | Isopropanol | 4fold activation by preincubation with 80%, v/v | Talaromyces funiculosus |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.21 | bovine serum albumin required for stabilizing the enzyme preparation | Talaromyces funiculosus |
| 3.2.1.21 | marked instability after purification | Talaromyces funiculosus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | beta-D-glucose | competitive inhibition, Ki: 0.9 mM | Talaromyces funiculosus | |
| 3.2.1.21 | D-glucono-1,5-lactone | competitive inhibition, Ki: 0.03 mM | Talaromyces funiculosus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.001 | - |
4-nitrophenyl-beta-D-glucopyranoside | isoform II | Talaromyces funiculosus | |
| 3.2.1.21 | 0.002 | - |
cellobiose | - |
Talaromyces funiculosus | |
| 3.2.1.21 | 0.002 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Talaromyces funiculosus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.21 | extracellular | absorption of enzyme to mycelium, if cells grown on glucose medium | Talaromyces funiculosus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 18000 | - |
gel filtration, isoform II | Talaromyces funiculosus |
| 3.2.1.21 | 120000 | - |
2 * 120000, SDS-PAGE, isoform I | Talaromyces funiculosus |
| 3.2.1.21 | 230000 | - |
gel filtration, isoform I | Talaromyces funiculosus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Talaromyces funiculosus | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Talaromyces funiculosus | gentiobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | cellopentaose + 4 H2O | Talaromyces funiculosus | - |
5 D-glucose | - |
? | |
| 3.2.1.21 | cellotetraose + 3 H2O | Talaromyces funiculosus | - |
4 D-glucose | - |
? | |
| 3.2.1.21 | cellotriose + 2 H2O | Talaromyces funiculosus | - |
3 D-glucose | - |
? | |
| 3.2.1.21 | laminarin + H2O | Talaromyces funiculosus | low activity | beta-D-glucose + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Talaromyces funiculosus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | glycoprotein | 10.7% carbohydrate content, w/w, isoform I | Talaromyces funiculosus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | to homogeneity, preparative electrophoresis, 2 isoforms | Talaromyces funiculosus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | cell culture | - |
Talaromyces funiculosus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.21 | -20°C, stable for months in solution | Talaromyces funiculosus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | low activity | Talaromyces funiculosus | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Talaromyces funiculosus | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Talaromyces funiculosus | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Talaromyces funiculosus | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Talaromyces funiculosus | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | cellopentaose + 4 H2O | - |
Talaromyces funiculosus | 5 D-glucose | - |
? | |
| 3.2.1.21 | cellotetraose + 3 H2O | - |
Talaromyces funiculosus | 4 D-glucose | - |
? | |
| 3.2.1.21 | cellotriose + 2 H2O | - |
Talaromyces funiculosus | 3 D-glucose | - |
? | |
| 3.2.1.21 | laminarin + H2O | low activity | Talaromyces funiculosus | beta-D-glucose + ? | - |
? | |
| 3.2.1.21 | methyl-beta-D-glucoside + H2O | very low activity | Talaromyces funiculosus | beta-D-glucose + methanol | - |
? | |
| 3.2.1.21 | phenyl-beta-D-glucoside + H2O | - |
Talaromyces funiculosus | phenol + beta-D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | dimer | 2 * 120000, SDS-PAGE, isoform I | Talaromyces funiculosus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
