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Literature summary extracted from
- Purification and characterization of a beta-glucosidase from Trichoderma reesei (1987), Eur. J. Biochem., 165, 333-341.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | beta-D-glucose | Ki: 0.7 mM | Trichoderma reesei |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.02 | - |
4-methylumbelliferyl-beta-D-glucoside | - |
Trichoderma reesei | |
| 3.2.1.21 | 0.102 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Trichoderma reesei | |
| 3.2.1.21 | 1.25 | - |
cellobiose | - |
Trichoderma reesei | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.21 | extracellular | - |
Trichoderma reesei | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 81600 | - |
SDS-PAGE | Trichoderma reesei |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Trichoderma reesei | cellobiose | beta-D-glucose + D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Trichoderma reesei | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | to homogeneity, chromatography steps | Trichoderma reesei |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | cell culture | - |
Trichoderma reesei | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 52 | - |
- |
Trichoderma reesei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-methylumbelliferyl-beta-D-glucoside + H2O | - |
Trichoderma reesei | 4-methylumbelliferone + beta-D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Trichoderma reesei | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Trichoderma reesei | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
Trichoderma reesei | 2 beta-D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | monomer | 1 * 81600, SDS-PAGE | Trichoderma reesei |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 4.5 | 5 | - |
Trichoderma reesei |
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