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Literature summary extracted from

  • Takase, M.; Horikoshi, K.
    A thermostable beta-glucosidase isolated from a bacterial species of the genus Thermus (1988), Appl. Microbiol. Biotechnol., 29, 55-60.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.28
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Thermus sp.
3.2.1.21 2
-
 cellobiose
-
 Thermus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 intracellular induction of enzyme synthesis by addition of cellobiose or laminaribiose to the medium Thermus sp. 5622
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Thermus sp. laminaribiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O Thermus sp. low activity beta-D-galactose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O Thermus sp. lactose beta-D-galactose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Thermus sp. cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O Thermus sp. low activity beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O Thermus sp. gentiobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellohexaose + 5 H2O Thermus sp. low activity 6 D-glucose
-
 ?
3.2.1.21 cellopentaose + 4 H2O Thermus sp.
-
 5 D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O Thermus sp.
-
 4 D-glucose
-
 ?
3.2.1.21 cellotriose + 2 H2O Thermus sp.
-
 3 D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Thermus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 partial Thermus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture
-
 Thermus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 11.34
-
 in cell-free extract for laminaribiose, maximum rate of all substrates tested Thermus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Thermus sp. beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermus sp. 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O low activity Thermus sp. beta-D-galactose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O lactose Thermus sp. beta-D-galactose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Thermus sp. beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O low activity Thermus sp. beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Thermus sp. beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Thermus sp. 2 beta-D-glucose
-
 ?
3.2.1.21 cellohexaose + 5 H2O low activity Thermus sp. 6 D-glucose
-
 ?
3.2.1.21 cellopentaose + 4 H2O
-
 Thermus sp. 5 D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O
-
 Thermus sp. 4 D-glucose
-
 ?
3.2.1.21 cellotriose + 2 H2O
-
 Thermus sp. 3 D-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 85
-
-
 Thermus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 75
-
 half-life: 5 days Thermus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 4.5 6.5 optimal activity within Thermus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 4.5 7 stable for 2 h at 70°C Thermus sp.