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Literature summary extracted from

  • Hayashida, S.; Ohta, K.; Mo, K.
    Cellulases of Humicola insolens and Humicola grisea (1988), Methods Enzymol., 160, 323-332.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.21 stable in the presence of urea Humicola insolens
3.2.1.21 stable in the presence of urea Trichocladium griseum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Cu2+ 55% of original activity inhibitited Humicola insolens
3.2.1.21 Cu2+ 55% of original activity inhibitited Trichocladium griseum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 extracellular
-
 Humicola insolens
-
-
3.2.1.21 extracellular
-
 Trichocladium griseum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 57000
-
 x * 57000, SDS-PAGE Humicola insolens
3.2.1.21 250000
-
 SDS-PAGE Humicola insolens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Humicola insolens cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Trichocladium griseum cellobiose beta-D-glucose + D-glucose
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.21 dimethyl sulfoxide significantly resistant even at high concentrations Humicola insolens
3.2.1.21 dimethyl sulfoxide significantly resistant even at high concentrations Trichocladium griseum
3.2.1.21 Ethanol significantly resistant even at high concentrations Humicola insolens
3.2.1.21 Ethanol significantly resistant even at high concentrations Trichocladium griseum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Humicola insolens
-
-
-
3.2.1.4 Trichocladium griseum
-
-
-
3.2.1.21 Humicola insolens
-
-
-
3.2.1.21 Trichocladium griseum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.4 glycoprotein contains 39% carbohydrate Humicola insolens
3.2.1.21 glycoprotein
-
 Trichocladium griseum
3.2.1.21 glycoprotein 1.7%, w/w, mannose, 0.8%, w/w, glucose residues Humicola insolens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Humicola insolens
3.2.1.4
-
 Trichocladium griseum
3.2.1.21 to homogeneity, 3step chromatography Humicola insolens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture
-
 Humicola insolens
-
3.2.1.21 cell culture
-
 Trichocladium griseum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 266.9
-
-
 Humicola insolens
3.2.1.21 266.9
-
-
 Trichocladium griseum

Storage Stability

EC Number Storage Stability Organism
3.2.1.4 0-4°C, 0.05 M acetate buffer, pH 5.0, stable for at least 2 weeks Humicola insolens
3.2.1.21 0-4°C, 0.05 M acetate buffer, pH 5.0, 2 weeks Humicola insolens
3.2.1.21 0-4°C, 0.05 M acetate buffer, pH 5.0, 2 weeks Trichocladium griseum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Humicola insolens 2-hydroxymethylphenol + beta-D-glucose
-
 ?
3.2.1.21 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Trichocladium griseum 2-hydroxymethylphenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Humicola insolens 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Trichocladium griseum 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Humicola insolens beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Trichocladium griseum beta-D-glucose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 57000, SDS-PAGE Humicola insolens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 50
-
-
 Humicola insolens
3.2.1.21 50
-
-
 Humicola insolens
3.2.1.21 50
-
-
 Trichocladium griseum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 65
-
 5 min, stable Humicola insolens
3.2.1.4 95
-
 5 min, inactivation Humicola insolens
3.2.1.21 60
-
 stable for 5 min Humicola insolens
3.2.1.21 60
-
 stable for 5 min Trichocladium griseum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5
-
-
 Humicola insolens
3.2.1.21 5
-
-
 Trichocladium griseum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 3 11 stable Humicola insolens
3.2.1.21 4 8 stable within Humicola insolens
3.2.1.21 4 8 stable within Trichocladium griseum